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Environmental lead exposure and its relationship to traffic density among Senegalese children: a cross-sectional study

Laboratoire de Chimie Analytique et Toxicologie, Faculté de Médecine et de Pharmacie, UCAD, Dakar, Senegal

G Garçon

LCE-EA2598, Toxicologie Industrielle et Environnementale, Université du Littoral? Côte d'Opale, Dunkerque, France

Y Diop

B Ndiaye

C Thiaw

M Fall

O Kane-Barry

D Ba

Laboratoire de Chimie Analytique et Toxicologie,Faculté de Médecine et de Pharmacie, UCAD, Dakar, Senegal

J M Haguenoer

Laboratoire de Toxicologie, Santé Publique et Environnement, Faculté des Sciences Pharmaceutiques et Biologiques, Lille, France; Laboratoire Universitaire de Médecine du Travail et Environnement, Faculté de Médecine, Lille, France

P Shirali

LCE-EA2598, Toxicologie Industrielle et Environnementale, Université du Littoral? Côte d'Opale, Dunkerque, France; LCE-EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France pirouz.shirali{at}univ-littoral.fr

Leaded-gasoline is probably the primary source of lead (Pb) exposure in Dakar (Senegal). The present cross sectional study was undertaken to investigate the levels of Pb in Senegalese children and to present helpful data on the relationship between Pb levels and changes in biological markers of heme biosynthesis and oxidative stress. A total of 330 children, living since birth either in rural or urban areas (ie, Khombole (n=162) and Dakar (n=168), respectively) were included. During this cross sectional study, the mean blood (B)-Pb level in all children was 7.32±5.33 ug/dL, and was influenced by the area of residence and gender. In rural children, 27 subjects (16.7%), 18 boys (19.6%) and nine girls (12.9%), had a B-Pb level >10 ug Pb/dL, whereas 99 urban children (58.9%), respectively, 66 boys (71.8%) and 33 girls (43.4%), had alarmingly high B-Pb levels. Accordingly, urine delta-aminolevulinic acid levels were higher in children living in the urban area than in the rural areas (P B±0.001), and closely correlated with the B-Pb levels (P B±0.01). Moreover, glutathione peroxidase (GPx) activity, selenium (Se) level, glutathione reductase (GR) activity, and glutathione status were significantly influenced by area of residence and/or by gender. GPx activity and Se level were not only negatively correlated with B-Pb levels, but also positively correlated together (P B±0.01). Taken together, the present results allow us to conclude that urban children have higher B-Pb levels than rural children, and that of these children, boys have higher B-Pb levels than girls, leading thereby to alterations of heme biosynthesis and pro-oxidant/antioxidant balance. We also suggest that exposure to Pb and the Pb-induced adverse effects merits attention and that the development of preventive actions are of increasing importance in Senegal.

Key Words: children • exposure • heme synthesis • lead • oxidative stress • traffic pollution

References

• Adonaylo VN, Oteiza PI. Lead intoxication: antioxidant defenses and oxidative damage in rat brain . Toxicology 1999; 135: 77–85 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Bayomi RA, Rosalki SB. Evaluation of methods of coenzyme activation of erythrocyte enzymes for detection of deficiency of vitamins B1, B2 and B6 . Clin Chem 1976; 22: 327–335 .[Abstract/Free Full Text]
• Bellinger D, Dietrich KN. Low-level lead exposure and cognitive functioning in children . Pediatr Ann 1994; 23: 600–605 .[Web of Science][Medline] [Order article via Infotrieve]
• Benes B, Spevackova V, Cejchanova M, Smid J, Svandova E. Retrospective study of concentration levels of Pb, Cd, Cu and Se in serum of the Czech population in time period 1970–1999 . Cent Eur J Publ Health 2001; 9: 190–195 .
• Burbure C, Buchet JP, Bernard A, Leroyer A, Nisse C, Haguenoer JM, Bergamaschi E, Mutti A. Biomarkers of renal effects in children and adults with low environmental exposure to heavy metals . J Toxicol Health A 2003; 66: 783–798 .[CrossRef]
• Campagna D, Huel G, Girarda F, Sahuquilloa J, Blot P. Environmental lead exposure and activity of d-aminolevulinic acid dehydratase (ALA-D) in maternal and cord blood . Toxicology 1999; 134: 143–152 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Centers for Disease Control and Prevention. Preventing lead poisoning in young children: a statement by the Centers for Disease Control and Prevention. Centers for Disease Control and Prevention , 1991.
• Diaz-Alcon JP, Navarron-Alarcon M, Lopez-Garcia de la Serrana H, Lopez-Martinez MC. Determination of selenium in cereals, legumes and dry fruits from southeastern Spain for calculation of daily dietary intake . Sci Total Environ 1996; 184: 183–189 .[CrossRef][Medline] [Order article via Infotrieve]
• Dietrich KN, Berger OG, Succop PA. Lead exposure and the motor developmental status of urban six-yearold children in the Cincinnati Prospective Study . Pediatrics 1994; 91: 301–307 .
• Ding Y, Gonick HC, Vaziri ND. Lead promotes hydroxyl radical generation and lipid peroxidation in cultured aortic endothelial cells . Am J Hypertens 2000; 13: 552–555 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Diouf A, Sarr MM, Niane B, Gueye A, Ba D, Ciss M. Air pollution by lead emitted from vehicles in the Dakar region . Dakar Med 1995; 40: 117–121 .[Medline] [Order article via Infotrieve]
• Diouf A, Garçon G, Thiaw C, Diop Y, Fall M, Ndiaye B, Siby T, Hannothiaux MH, Zerimech F, Ba D, Haguenoer JM, Shirali P. Environmental lead exposure and its relationship to traffic density among Senegalese children: a pilot study . Hum Exp Toxicol 2003; 22: 559–564 .[Abstract/Free Full Text]
• Ercal N, Neal R, Treeratphan P, Lutz PM, Hammond TC, Dennery PA, Spitz DR. A role for oxidative stress in suppressing serum immunoglobulin levels in lead exposed Fisher 344 rats . Arch Environ Contam Toxicol 2000; 39: 251–256 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Fels LM, Wansch M, Baranowski J, Norska-Borawka I, Price RG, Taylor SA, Patel S, De Broe M, Elsevier MM, Lauwerys R, Roels H, Bernard A, Mutti A, Gelpi E, Rosella J, Stolte H. Adverse effects of chronic low level lead exposure on kidney function–a risk group study in children . Nephrol Dial Transplant 1998; 13: 2248–2256 .[Abstract/Free Full Text]
• Garçon G, Garry S, Gosset P, Zerimech F, Martin A, Hannothiaux MH, Shirali P. Benzo(a)pyrene-coated onto Fe2O3 particles-induced lung tissue injury: role of free radicals . Cancer Lett 2001; 167: 7–15 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Garçon G, Leleu B, Zerimech F, Marez T, Haguenoer JM, Furon D, Shirali P. Biological markers of oxidative stress and nephrotoxicity as studied in biomonitoring of adverse effects of occupational exposure to lead and cadmium . J Occup Env Med 2004; 46; 1180–1186 .[Web of Science][Medline] [Order article via Infotrieve]
• Goyer RA. Toxic effects of metals. In Amdur MO, Doull J, Klaassen CD eds. Casarett and Doull's toxicology, fourth edition. McGraw-Hill , 1991: 642–643.
• Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Rad Biol Med 2000; 7: 927–945 .
• Gurer-Ohran H, Sabir HU, Ozgunes H. Correlation between clinical indicators of lead poisoning and oxidative stress parameters in controls and lead exposed workers . Toxicology 2004; 195: 147–154 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Heinze I, Gross R, Stehle P, Dillon D. Assessment of lead exposure in schoolchildren from Jakarta . Environ Health Perspect 1998; 106: 499–501 .[Web of Science][Medline] [Order article via Infotrieve]
• Hsu PC, Guo YL. Antioxidant nutrients and lead toxicity . Toxicology 2002; 180: 33–44 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Lanphear BP, Dietrich K, Auinger P, Cox C. Cognitive deficits associated with blood lead concentrations B 10 mg/dL in US children and adolescents . Publ Health Rep 2000; 115: 521–529 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Lee BK, Ahn KD, Lee SS, Lee GS, Kim YB, Schwartz BS. A comparison of different lead biomarkers in their associations with lead-related symptoms . Int Arch Occup Environ Health 2000; 73: 298–304 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Malcoe LH, Lynch RA, Keger MC, Skaggs VJ. Lead sources, behaviors, and socioeconomic factors in relation to blood lead of native American and white children: a community-based assessment of a former mining area . Environ Health Perspect 2002; 110: 221–231 .[Web of Science][Medline] [Order article via Infotrieve]
• Massadeh AM, Snook RD. Determination of Pb and Cd in road dusts over the period in which Pb was removed from petrol in the UK . J Environ Monit 2002; 4: 567–572 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• McCord JM, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (hemocyprein) . J Biol Chem 1969; 244: 6049–6055 .[Abstract/Free Full Text]
• Moss ME, Lanphear BP, Auinger P. Association of dental caries and blood lead levels . JAMA 1999; 281: 2294–2298 .[Abstract/Free Full Text]
• Neal R, Cooper K, Kellogg G, Gurer H, Ercal N. Effect of some sulfur-containing antioxidants on lead-exposed lenses . Free Rad Biol Med 1999; 26: 239–243 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Needleman HL, Schell A, Bellinger D, Leviton A, Allred EN. The long-term effects of exposure to low doses of lead in childhood: an 11-year follow-up report . New Engl J Med 1990; 322: 83–88 .[Abstract]
• Odukoya OO, Arowolo TA, Bamgbose O. Pb, Zn, and Cu levels in tree barks as indicator of atmospheric pollution . Environ Int 2000; 26: 11–16 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Oktem F, Arslan MK, Dundar B, Delibas N, Gultepe M, Ergurhan Ilhan I. Renal effects and erythrocyte oxidative stress in long-term low-level lead-exposed adolescent workers in auto repair workshops . Arch Toxicol 2004; 78: 681–687 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase . J Lab Clin Med 1967; 70: 158-158 .[Web of Science][Medline] [Order article via Infotrieve]
• Schutz A, Barregard L, Sallsten G, Wilske J, Manay N, Perreira L, Cousillas Z. Blood lead in Uruguayan children and possible sources of exposure . Environ Res 1997; 74: 17–23 .[Medline] [Order article via Infotrieve]
• Schumhacher M, Belles M, Rico A, Domingo JL, Corbella J. Impact of reduction of lead in gasoline on the blood and hair lead levels in the population of Tarragona province, Spain, 1990–1995 . Sci Total Environ 1999; 183: 203–209 .
• Schwartz J. Low-level lead exposure and children's IQ: a meta-analysis and search for a threshold . Environ Res 1994; 65: 42–55 .[Medline] [Order article via Infotrieve]
• Schwartz J, Otto D. Lead and minor hearing impairment . Arch Environ Health 1991; 46: 300–305 .[Web of Science][Medline] [Order article via Infotrieve]
• Shaltout AA, Guthrie R, Moussa M, Kandil H, Hassan MF, Dosari L, Hunt MCJ, Fernando NP. Erythrocyte protoporphyrin screening for lead poisoning in Bedouin children. A study from Kuwait . J Trop Pediat 1989; 35: 87–91 .[Abstract/Free Full Text]
• Tomokuni K. New method for determination of aminolevulinate dehydratase activity of human erythrocytes as an index of lead exposure . Clin Chem 1994; 20: 1287–1291 .
• Van Kampen EJ, Zijlstra WG. Standardization of hemoglobinometry. II. The hemoglobincyanid method . Clin Chim Acta 1961; 6: 538–544 .[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
• Verberk MM, Willems TE, Verplanke AJ, De Wolff FA. Environmental lead and renal effects in children . Arch Environ Health 1996; 51: 83–87 .[Web of Science][Medline] [Order article via Infotrieve]
• Von Schirnding YER, Fuggle RF. A study of the distribution of urban environmental lead levels in Cape Town, South Africa . Sci Total Environ 1996; 187: 1–8 .[CrossRef]
• Wasserman GA, Liu X, Lolaconno NJ, Factor-Litvak P, Kline JK, Popovac D, Morina N, Musabegovic A, Vrenezi N, Capuni-Paracka S. Lead exposure and intelligence in 7-year-old children: the Yugoslavia Prospective Study . Environ Health Perspect 1997; 105: 956–962 .[Web of Science][Medline] [Order article via Infotrieve]
• Wilhelm M, Pesch A, Rostek U, Begerow J, Scmitz N, Idel H, Ranft U. Concentrations of lead in blood, hair and saliva of German children living in three different areas of traffic density . Sci Total Environ 2002; 297: 109–118 .[CrossRef][Medline] [Order article via Infotrieve]
• Xie Y, Chiba M, Shinohara A, Watanabe H, Inaba Y. Studies on lead-binding protein and interaction between lead and selenium in the human erythrocytes . Ind Health 1998; 36: 234–239 .[Web of Science][Medline] [Order article via Infotrieve]
• Zejda JE, Grabecki J, Krol B, Jedrezejczak A, Jarkowski M. Blood lead levels in urban children of Katowice Voivodoship, Poland: results of the population-based biomonitoring and surveillance program . Centr Eur J Publ Health 1997; 2: 60–64 .

Human & Experimental Toxicology, Vol. 25, No. 11, 637-644 (2006)
DOI: 10.1177/0960327106074591


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