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Effect of Nicotine and Cotinine on the Production of Oxygen Free Radicals by Neutrophils in Smokers and Non-smokers

Department of Gastroenterology, University Hospital of Wales, Cardiff

M.B. Hallett

Department of Surgery, University Hospital of Wales, Cardiff

J. Rhodes

Department of Gastroenterology, University Hospital of Wales, Cardiff

1 Neutrophils play a role in the pathogenesis of ulcerative colitis. Most of the patients with ulcerative colitis are non-smokers.

2 Neutrophil function was examined in healthy males, five smokers and five non-smokers, measuring production of oxygen free radicals by chemiluminescence.

3 The chemiluminescence was examined in the presence of nicotine and its main metabolite cotinine in various concentrations.

4 10 mM nicotine inhibited production of oxygen free radicals (90.2% ± 11.6) compared with controls (P < 0.001), as did the same concentration of cotinine (58.9% ± 34.2), (P < 0.05).

5 1 mM solutions had no effect and 3 mM nicotine was required to achieve 50% inhibition.

6 Smokers and non-smokers did not differ.

7 Since plasma concentrations of nicotine after a cigarette are only between 5 and 15 mM, the observed effects of nicotine and cotinine in much higher pharmacological concentrations in this system are unlikely to be of clinical relevance in ulcerative colitis

Human & Experimental Toxicology, Vol. 8, No. 6, 461-463 (1989)
DOI: 10.1177/096032718900800605


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