This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Rao, B. Articles by Kumar, M. Search for Related Content PubMed PubMed Citation Articles by Rao, B. Articles by Kumar, M. Social Bookmarking                         What's this?

Preventive efficacy of hydroalcoholic extract of Cymbopogon citratus against radiation-induced DNA damage on V79 cells and free radical scavenging ability against radicals generated in vitro

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, Indiasatishraomlsc{at}gmail.com

R Shanbhoge

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India

BN Rao

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India

SK Adiga

Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Hospital, Manipal University, Manipal, Karnataka, India

D Upadhya

Division of Reproductive Medicine, Department of Obstetrics and Gynecology, Kasturba Hospital, Manipal University, Manipal, Karnataka, India

BK Aithal

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India

MRS Kumar

Division of Radiobiology & Toxicology, Manipal Life Sciences Center, Manipal University, Manipal, Karnataka, India

This study presents the findings of free radical scavenging and antigenotoxic effect of hydroalcoholic extract of Cymbopogon citratus (CCE). The CCE at a concentration of 60 µg/mL resulted in a significant scavenging ability of 2,2-diphenyl-2-picryl hydrazyl (DPPH; (85%), 2,2-azinobis (3-ethyl benzothiazoline-6-sulphonic acid) (ABTS; 77%), hydroxyl (70%), superoxide (76%), nitric oxide (78%) free radicals generated using in vitro and also a moderate anti-lipid peroxidative effect (57%). Further, the radiation-induced antigenotoxic potential of CCE was assessed in Chinese hamster lung fibroblast cells (V79) using micronucleus assay. The CCE resulted in a dose-dependent decrease in the yield of radiation-induced micronuclei, with a maximum effect at 125 µg/mL CCE for 1 h before 2 Gy of radiation. Similarly, there was a significant (P < 0.05–0.0001) decrease in percentage of micronuclei when V79 cells were treated with optimal dose of CCE (125 µg/mL) before exposure to different doses of gamma radiation, that is, 0.5–4 Gy, compared with radiation alone groups. The results of the micronucleus study indicated antigenotoxic effect demonstrating the radioprotective potential of CCE and, which may partly due to its and antioxidant capacity as it presented its ability to scavenge various free radicals in vitro and anti-lipid peroxidative potential.

Key Words: antioxidant • Cymbopogon citratus • free radical scavenger • micronuclei • radioprotection

Human & Experimental Toxicology, Vol. 28, No. 4, 195-202 (2009)
DOI: 10.1177/0960327109104822


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?