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Human & Experimental Toxicology
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Effect of m-3M3FBS on Ca2+ movement in Madin-Darby canine renal tubular cells

Yi-Chien Fang

Laboratory Medicine Division, Zuoying Armed Forces General Hospital, Kaohsiung, Taiwan, Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan

Daih-Huang Kuo

Department of Pharmacy, Tajen University, Pingtung, Taiwan

Pochuen Shieh

Department of Pharmacy, Tajen University, Pingtung, Taiwan

Fu-An Chen

Department of Pharmacy, Tajen University, Pingtung, Taiwan

Chun-Chi Kuo

Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan, Department of Nursing, Tzu Hui Institute of Technology, Pingtung, Taiwan

Chung-Ren Jan

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, crjan{at}isca.vghks.gov.tw

The effect of 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzenesulfonamide (m-3M3FBS), a presumed phospholipase C (PLC) activator, on cytosolic free Ca2+ concentrations ([Ca 2+]i) in Madin-Darby canine kidney (MDCK) cells is unclear. This study explored whether m-3M3FBS changed basal [Ca2+]i levels in suspended MDCK cells using fura-2 as a Ca2+-sensitive fluorescent dye. M-3M3FBS at concentrations between 0.1 and 20 µM increased [Ca2+]i in a concentration-dependent manner. The Ca2+ signal was decreased by removing extracellular Ca2+. M-3M3FBS-induced Ca2+ influx was inhibited by the store-operated Ca2+ channel blockers nifedipine, econazole, and SK&F96365, and by the phospholipase A2 inhibitor aristolochic acid. In Ca2+-free medium, 20-µM m-3M3FBS pretreatment abolished the [Ca2+]i rise induced by the endoplasmic reticulum Ca2+ pump inhibitors thapsigargin (TG) and cyclopiazonic acid (CPA). Conversely, pretreatment with TG or CPA partly reduced m-3M3FBS-induced [Ca2+]i rise. The inhibition of PLC with U73122 did not alter m-3M3FBS-induced [Ca2+]i rise. Collectively, in MDCK cells, m-3M3FBS induced [Ca2+]i rises by causing PLC-independent Ca2+ release from the endoplasmic reticulum and Ca2+ influx via store-operated Ca2+ channels and other unidentified Ca2+ channels.

Key Words: Ca2+ • m-3M3FBS • MDCK • renal cells

This version was published on October 1, 2009

Human & Experimental Toxicology, Vol. 28, No. 10, 655-663 (2009)
DOI: 10.1177/0960327109106972
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