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A nutrient mixture suppresses carbon tetrachloride–induced acute hepatic toxicity in ICR mice

Dr Rath Research Institute, Santa Clara, California, USA

T Kalinovsky

Dr Rath Research Institute, Santa Clara, California, USA

NW Roomi

Dr Rath Research Institute, Santa Clara, California, USA

V Ivanov

Dr Rath Research Institute, Santa Clara, California, USA

M Rath

Dr Rath Research Institute, Santa Clara, California, USA

A Niedzwiecki

Dr Rath Research Institute, Santa Clara, California, USA author{at}drrath.com

We examined the effect of a nutrient mixture (NM) that contains lysine, proline, ascorbic acid, and green tea extract in mice treated with carbon tetrachloride (CCl₄), a model of liver injury in which free radical, oxidative stress, and cytokine production are closely linked. Seven-week-old male Imprinting Control Region (ICR) mice were divided into four groups (A–D) of five animals each. Groups A and C mice were fed a regular diet for 2 weeks, whereas groups B and D mice were supplemented with 0.5% NM (w/w) during that period. Groups A and B received corn oil i.p., whereas groups C and D received CCl₄ (25 µL/kg, in corn oil, i.p.). All animals were killed 24 h after CCl₄ administration, serum was collected to assess liver and kidney functions, and livers and kidneys were excised for histology. Mean serum aspartate aminotransferase and alanine aminotransferase were comparable in groups A and B, increased markedly in group C, and significantly lowered in group D compared with group C. CCl₄ had no significant effect on renal markers (blood urea nitrogen [BUN], creatinine, and BUN/creatinine ratio). CCl₄ administration caused an intense degree of liver necrosis that was less severe in the NM fed group D. These results indicate that NM could be a useful supplement in preventing acute chemical-induced liver toxicity.

Key Words: carbon tetrachloride • hepatic • toxicity • nutrients • ICR mice

Human & Experimental Toxicology, Vol. 27, No. 7, 559-566 (2008)
DOI: 10.1177/0960327108096851


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