This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kaymak, C Articles by Sardas, S Search for Related Content PubMed PubMed Citation Articles by Kaymak, C Articles by Sardas, S Social Bookmarking                         What's this?

Oxidative DNA damage and total antioxidant status in rats during experimental gram-negative sepsis

Faculty of Medicine, Department of Anesthesiology and Reanimation, Kirikkale University, Kirikkale, Turkey

E Kadioglu

Faculty of Pharmacy, Department of Toxicology, Gazi University, Ankara, Turkey

E Ozcagli

Faculty of Pharmacy, Department of Toxicology, Gazi University, Ankara, Turkey

G Osmanoglu

Faculty of Medicine, Department of General Surgery, Kirikkale University, Kirikkale, Turkey

S Izdes

Department of Anesthesiology and Reanimation, Ataturk Training and Research Hospital, Ministry of Health, Ankara, Turkey

C Agalar

Faculty of Medicine, Department of Infection Diseases, Kirikkale University, Kirikkale, Turkey

H Basar

Department of Anesthesiology and Reanimation, Ankara Training and Research Hospital, Ministry of Health, Ankara, Turkey

S Sardas

Faculty of Pharmacy, Department of Toxicology, Marmara University, Istanbul, Turkey semrasardas{at}gmail.com

Sepsis and septic shock remains as leading cause of death in adult intensive care units. It is widely accepted that gram-negative bacteria and their endotoxins cause sepsis and septic shock, predominantly. Enhanced generation of reactive oxygen species may be responsible for tissue injury in septic shock and endotoxemia. The aim of this study was to assess oxidative DNA damage and the total antioxidant status (TAS) in peripheral lymphocytes of rats during different intraperitoneal gram-negative sepsis stages. Adult male Sprague-Dawley rats were divided randomly into four groups. Control group was intraperitoneally inoculated with 2 mL of pyrogene-free saline (Group I, n = 6), and the other rats received an intraperitoneal inoculum with 2 mL of saline containing 2 x 10⁸ CFU of Escherichia coli. The animals were killed at time zero (Group I, n = 6), at 6th (Group II, n = 7), 12th (Group III, n = 7), and 24th (Group IV, n = 7) hour after the E. coli inoculation. Oxidative DNA damage in peripheral lymphocytes of rats was evaluated by modified comet assay (single-cell gel electrophoresis). Formamidopyrimidine DNA glycosylase (Fpg) and Endonuclease III (Endo III) were used to detect oxidized purines and pyrimidines, respectively. Total antioxidant quantification was carried out using ABTS+ (2,2'-Azino-di-[3 ethylbenzthiazoline sulphonate]) radical formation kinetics (Randox kit) in serum samples. Significant elevations of basal levels of strand breaks (SB) in Group IV were observed as compared with Group I, II, and III. There was a significant increase in Fpg sites in Group III as compared with Group I and II. However, there was no significant difference in terms of Endo III sites in any of the groups. Although the TAS was decreased with the stages of sepsis, this moderate decrease was significant in only Group IV as compared with Group I. There was no statistically significant correlation between DNA damage and TAS for any of the groups.

Key Words: modified comet assay • oxidative DNA damage • sepsis • total antioxidant status

Human & Experimental Toxicology, Vol. 27, No. 6, 485-491 (2008)
DOI: 10.1177/0960327108088972


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?