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Human & Experimental Toxicology
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research-article

Toxicity of repeated intravenous injection of gene therapeutics for X-CGD in mice

YM Lee

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea

WH Choi

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea

YB Kim

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea

CS Ha

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea

CW Song

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea

M Lee

Department of Biology, University of Incheon, Incheon, Korea

CW Joo

Research and Development Center, ViroMed, Kwanak-gu, Seoul, Korea

Y Hong

Research and Development Center, ViroMed, Kwanak-gu, Seoul, Korea

SH Ho

Research and Development Center, ViroMed, Kwanak-gu, Seoul, Korea

S Kim

Research and Development Center, ViroMed, Kwanak-gu, Seoul, Korea

JM Kim

Research and Development Center, ViroMed, Kwanak-gu, Seoul, Korea

WS Koh

Department of Nonclinical Studies, Korea Institute of Toxicology, Yusung-gu, Daejeon, Korea wskoh{at}kitox.re.kr

We made gene therapeutics for X-chronic granulomatous disease (CGD) by transducing murine bone marrow-derived stem cells with MT-gp91 retrovirus and evaluated possible toxicity in mice as a prerequisite for human clinical trials. Male C57BL/6 mice were injected intravenously with gene therapeutics for X-CGD twice at an interval of two weeks at 5 x 107 cells/kg and sacrificed 2 weeks after the last administration. Significant changes noted in gene therapeutics for X-CGD-treated animals were an increase in white blood cell counts and a slight decrease in albumin/globulin ratio. The red pulp hyperplasia in the spleen accompanied with an increase in organ weight was considered to result from the accumulation of gene therapeutics for X-CGD, bone marrow-derived stem cells, in the spleen. No anti-gp91 antibody was detected in the sera collected from the animals treated with gene therapeutics for X-CGD. No integration of gp91 DNA from retroviral vector was detected in chromosomal DNA of gonads in animals dosed with the test substance, indicating no potential of genomic integration. In conclusion, the repeated dose of gene therapeutics for X-CGD exerted no toxicity. The splenic red pulp hyperplasia and the increase observed in white blood cell counts and in spleen weights were considered as pharmacological changes induced by the treatment.

Key Words: chromosomal integration • gene therapeutics • immunogenicity • toxicity

Human & Experimental Toxicology, Vol. 27, No. 5, 401-407 (2008)
DOI: 10.1177/0960327108094611
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