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Effect of melatonin, curcumin, quercetin, and resveratrol on acute ferric nitrilotriacetate (Fe-NTA)-induced renal oxidative damage in rats

Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Karlovarská 48, 301 66 Pilsen, Czech Republic vladislav.eybl{at}lfp.cuni.cz

D Kotyzová

Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Karlovarská 48, 301 66 Pilsen, Czech Republic

P erná

Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Karlovarská 48, 301 66 Pilsen, Czech Republic

J Koutensk

Department of Pharmacology and Toxicology, Charles University in Prague, Faculty of Medicine in Pilsen, Karlovarská 48, 301 66 Pilsen, Czech Republic

The influence of melatonin, curcumin, quercetin, and resveratrol pretreatment on ferric nitrilotriacetate (Fe-NTA)-induced oxidative renal damage was studied. Male Wistar rats were treated orally once daily for 3 days with melatonin (10 mg/kg), curcumin (50 mg/kg), quercetin (15 mg/kg), and resveratrol (10 mg/kg). One hour after the last dose of antioxidants, a single dose of Fe-NTA was administered (8 mg of Fe/kg body weight, i.p.) to pre-treated animals. Twenty-four hours after Fe-NTA administration, the lipid peroxidation (LP), reduced glutathione (GSH), catalase (CAT), and glutathione peroxidase (GSH-Px) were estimated in kidney homogenates. Iron, zinc, and copper concentrations were estimated in kidney tissue. Administration of Fe-NTA to rats induced renal LP (170%, P < 0.001) and inhibited catalase (78%, P < 0.05) in the kidney. The oral pretreatment with melatonin, curcumin, quercetin, and resveratrol each one was effective in decreasing the Fe-NTA-induced LP (P < 0.001); however, it did not influence the FeNTA-induced inhibition of renal CAT activity. No changes were found in renal GSH level and GSH-Px activity compared to control animals. The pretreatment with antioxidants did not affect the increase in renal iron content, blood urea nitrogen/creatinine ratio, and relative kidney weight of FeNTA-intoxicated rats. The results indicate that the pretreatment with natural antioxidants, curcumin, melatonin, quercetin, and resveratrol, significantly and equally suppressed lipid peroxidation induced by Fe-NTA but had no effect on other markers of FeNTA nephrotoxicity and iron deposition in kidneys.

Key Words: curcumin • ferric nitrilotriacetate • resveratrol • renal oxidative damage • melatonin • quercetin

Human & Experimental Toxicology, Vol. 27, No. 4, 347-353 (2008)
DOI: 10.1177/0960327108094508


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