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Effects of calcium gluconate and PMSF in the treatment of acute intoxication of chicken by TOCP

Pharmaceutical Sciences – Graduation Program. School of Pharmaceutical Sciences UNESP-São Paulo State University, São Paulo, Brazil

RGP Machado

Department of Natural Active Principles and Toxicology. School of Pharmaceutical Sciences UNESP São Paulo State University, São Paulo, Brazil

G Honorato de Oliveira

Pharmaceutical Sciences – Graduation Program. School of Pharmaceutical Sciences UNESP-São Paulo State University, São Paulo, Brazil; Department of Natural Active Principles and Toxicology. School of Pharmaceutical Sciences UNESP São Paulo State University, São Paulo, Brazil gholive{at}fcfar.unesp.br

To examine the efficacy of calcium gluconate (two doses of Ca-Glu 5 mg/kg i.v.) to alleviate the injurious effects of organophosphorus induced delayed neuropathy (OPIDN) in the presence or absence of phenylmethanesulfonyl fluoride (PMSF 90 mg/kg i.m.), 14 groups of four isabrown hens were used. To measure the lymphocyte neuropathy target esterase (LNTE)activity, groups receiving just distilled water (control), groups receiving just Tri-orto-cresyl phosphate (TOCP; 500 mg/kg p.o.) (Positive control), and other groups receiving TOCP and Ca-Glu or PMSF simultaneously or 12 hours later following intoxication by TOCP were used. They were sacrificed 12 and 24 hours after the administration of TOCP. To observe a 28-day time course of neurotoxicity scores and calcium plasma concentration, five groups were used. Regarding free Ca²⁺in the plasma, the positive control produced a characteristic profile time course up and down during 28 days, and some hens with maximum score of neurotoxicity in 28 days. The treatment, which prevented greater oscillation in free Ca²⁺ in the plasma, presented a decrease in OPIDN in relation to the positive control. Twelve hours after the administration of TOCP, LNTE was 70–80% inhibited when compared with control, whereas the first decrease in the free Ca²⁺ in the plasma was significantly different from the control only 24 hours after the administration of TOCP. In summary, the sooner the Ca-Glu is started, the less severe the neuropathy effects.

Key Words: calcium gluconate • chicken • neurophathy target esterase • organophasphate-induced delayed neuropathy • tri-orto-cresyl phosphate • phenylmethanesulfonyl fluoride •

Human & Experimental Toxicology, Vol. 27, No. 3, 247-252 (2008)
DOI: 10.1177/0960327108090273


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