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Transcriptional changes in U343 MG-a glioblastoma cell line exposed to ionizing radiation

Department of Genetics

SS Mello

Department of Genetics

RS Cardoso

Department of Genetics

PDV Godoy

Department of Genetics; Department of Surgery and Anatomy; Department of Hematology; Department of Clinical Medicine; Faculty of Medicine (FMRP-USP), University of Sao Paulo (USP); Department of Morphology, Faculty of Dentistry (FORP-USP), Ribeirao Preto, SP, Brazil; Department of Biology, Faculty of Philosophy, Sciences and Letters (FFCLRP-USP), University of Sao Paulo, Ribeirao Preto, SP, Brazil

AL Fachin

Department of Genetics

CM Junta

Department of Genetics

P Sandrin-Garcia

Department of Genetics

CG Carlotti

Department of Surgery and Anatomy

RP Falcão

Department of Hematology

EA Donadi

Department of Clinical Medicine; Faculty of Medicine (FMRP-USP), University of Sao Paulo (USP)

GAS Passos

Department of Genetics; Department of Morphology, Faculty of Dentistry (FORP-USP), Ribeirao Preto, SP, Brazil

ET Sakamoto-Hojo

Department of Genetics; Department of Biology, Faculty of Philosophy, Sciences and Letters (FFCLRP-USP), University of Sao Paulo, Ribeirao Preto, SP, Brazil

Glioblastoma multiforme (GBM) is a highly invasive and radioresistant brain tumor. Aiming to study how glioma cells respond to -rays in terms of biological processes involved in cellular responses, we performed experiments at cellular context and gene expression analysis in U343-MG-a GBM cells irradiated with 1 Gy and collected at 6 h post-irradiation. The survival rate was approximately 61% for 1 Gy and was completely reduced at 16 Gy. By performing the microarray technique, 859 cDNA clones were analyzed. The Significance Analysis of Microarray algorithm indicated 196 significant expressed genes (false discovery rate (FDR) = 0.42%): 67 down-regulated and 97 up-regulated genes, which belong to several classes: metabolism, adhesion/cytoskeleton, signal transduction, cell cycle/apoptosis, membrane transport, DNA repair/DNA damage signaling, transcription factor, intracellular signaling, and RNA processing. Differential expression patterns of five selected genes (HSPA9B, INPP5A, PIP5K1A, FANCG, and TPP2) observed by the microarray analysis were further confirmed by the quantitative real time RT-PCR method, which demonstrated an up-regulation status of those genes. These results indicate a broad spectrum of biological processes (which may reflect the radio-resistance of U343 cells) that were altered in irradiated glioma cells, so as to guarantee cell survival.

Key Words: gene expression profiles • glioma cells • ionizing radiation • stress response

Human & Experimental Toxicology, Vol. 27, No. 12, 919-929 (2008)
DOI: 10.1177/0960327108102045


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