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Effects of the probiotic agent Saccharomyces Boulardii on the DNA damage in acute necrotizing pancreatitis induced rats

Department of Surgery, Gazi University Medical School, Ankara, Turkey

Sevtap Aydin

Department of Pharmaceutical Toxicology, Hacettepe University Faculty of Pharmacy, Ankara, Turkey

Osman Yüksel

Department of Surgery, Gazi University Medical School, Ankara, Turkey

Hasan Bostanci

Department of Surgery, Gazi University Medical School, Ankara, Turkey

Nalan Akyürek

Department of Pathology, Gazi University Medical School, Ankara, Turkey

Leyla Memis

Department of Pathology, Gazi University Medical School, Ankara, Turkey

Nursen Basaran

Department of Pharmaceutical Toxicology, Hacettepe University Faculty of Pharmacy, Ankara, Turkey, nbasaran{at}hacettepe.edu.tr

Pancreatitis is a mild and self-limiting disease. Although severe forms such as acute necrotizing pancreatitis (ANP) are rare it is associated with significant mortality rate reported to be 30—70%. Probiotics are viable microbial dietary supplements when introduced in sufficient quantities can have beneficial effects. The physiological effects of probiotics include suppression of bacterial infections, production of some digestive enzymes and vitamins and reconstruction of normal intestinal microflora. In the present study, the aim was to investigate the role of probiotics on the DNA damage in the peripheral lymphocytes, in the exfoliated epithelial cells and lymphocytes of the peritoneal fluids and in the pancreatic acinar cells of ANP induced rats. DNA damage was determined by COMET assay. ANP was induced by intravenous infusion of cerulein and superimposed infusion glycodeoxycholic acid into biliopancreatic duct. Saccharomyces Boulardii was used as the probiotic agent. DNA damage in pancreatic acinar cells and exfoliated epithelial cells and the lymphocytes of the peritoneal fluids; was significantly higher in pancreatitis group compared to the controls and probiotic treated groups (P<0.001). No significant difference was observed in the DNA damage between the groups in the peripheral lymphocytes. In conclusion; our results support that probiotic agent Saccharomyces Boulardii can diminish bacterial infections and offer health benefits in the therapy of pancreatitis. Human & Experimental Technololgy (2007) 26, 653—661

Key Words: acute necrotizing pancreatitis • COMET • DNA damage • probiotics

Human & Experimental Toxicology, Vol. 26, No. 8, 653-661 (2007)
DOI: 10.1177/0960327107077596


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