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Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C

Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey, mguney{at}med.sdu.edu.tr

Hilmi Demirin

Department of Biochemistry and Clinical Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

Baha Oral

Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

Meltem Özgüner

Department of Histology and Embryology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

Gökhan Bayhan

Department of Obstetrics and Gynecology, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

Irfan Altuntas

Department of Biochemistry and Clinical Biochemistry, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

We have investigated the effect of subchronic administration of methidathion (MD) on ovary evaluated ameliorating effects of vitamins E and C against MD toxicity. Experimental groups were as follows: control group; a group treated with 5 mg/kg body weight MD (MD group); and a group treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD + Vit group). MD and MD + Vit groups were given MD by gavage five days a week for four weeks at a dose level of 5 mg/kg/day by using corn oil as the vehicle. Serum malondialdehyde (MDA: an indicator of lipid peroxidation) concentration, serum activity of cholinesterase (ChE), and ovary histopathology were studied. The level of MDA increased significantly in the MD group compared with the control (P < 0.005). Serum MDA decreased significantly in the MD + Vit group compared with the MD group (P < 0.05). The activities of ChE decreased significantly both in the MD and MD + Vit groups compared with the controls ( P < 0.05). However, the decrease in the MD + Vit groups was less than in the MD group; the ChE activity in the MD + Vit group was significantly higher compared with MD group (P < 0.05). Number of ovarian follicles were significantly lower in the MD group compared to the controls (P < 0.05). Number of atretic follicles were significantly higher in the MD group than in the controls (P < 0.05). Follicle counts in MD + Vit group showed that all types of ovarian follicles were significantly higher, and a significant decrease in the number of atretic follicles compared with the MD group (P < 0.05). In conclusion, subchronic MD administration caused an ovarian damage, in addition, LPO may be one of the molecular mechanisms involved in MD-induced toxicity. Treatment with vitamins E and C after the administration of MD reduced LPO and ovarian damage. Human & Experimental Toxicology (2007) 26, 491—498

Key Words: lipid peroxidation • methidathion • ovarian toxicant • vitamin C • vitamin E

Human & Experimental Toxicology, Vol. 26, No. 6, 491-498 (2007)
DOI: 10.1177/0960327106077505


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