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Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil

Joel Ferreira Santiago Junior

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil

Flavia Mauad Levy

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil

Andrea Gutierrez Maria

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil

Mileni da Silva Fernandes

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil

Daisy Maria Favero Salvadori

Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, SP, Brazil

Daniel Araki Ribeiro

Department of Pathology, Botucatu Medical School, São Paulo State University, UNESP, SP, Brazil, Department of Health Sciences, Federal University of São Paulo, UNIFESP, Santos, SP, Brazil

Marilia Afonso Rabelo Buzalaf

Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, SP, Brazil, mbuzalaf{at}fob.usp.br

Fluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. In the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. The level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure. Human & Experimental Toxicology (2007) 26, 435—440

Key Words: acute exposure • comet assay • DNA damage • fluoride • rats

Human & Experimental Toxicology, Vol. 26, No. 5, 435-440 (2007)
DOI: 10.1177/0960327107076288


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