This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Baek, S Y Articles by Kim, Y Search for Related Content PubMed PubMed Citation Articles by Baek, S Y Articles by Kim, Y Social Bookmarking                   What's this?

Manganese does not alter the severe neurotoxicity of MPTP

Department of Anatomy, Pusan National University Medical School, Busan, South Korea

Y H Kim

Department of Neuropsychiatry, School of Medicine and Paik Institute of Clinical Research, Inje University, Busan, South Korea

S O Oh

Department of Anatomy, Pusan National University Medical School, Busan, South Korea

C-R Lee

C I Yoo

J H Lee

H Lee

C S Sim

Department of Occupational and Environmental Medicine, Ulsan University Hospital, College of Medicine, University of Ulsan, Ulsan, South Korea

J Park

Occupational Safety and Health Research Institute, Incheon, South Korea

J-W Kim

Department of Neurology, Dong-A University School of Medicine, Busan, South Korea

C S Yoon

Department of Occupational Health, Catholic University of Daegu, Gyeongbuk, South Korea

Y Kim

Department of Occupational and Environmental Medicine, Ulsan University Hospital, College of Medicine, University of Ulsan, Ulsan, South Korea

We utilized a mice model of Parkinsonism: (1) to evaluate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced neurotoxicity; and (2) to evaluate whether manganese (Mn) exposure can affect MPTP-induced neurotoxicity. A 2 x 3 experimental design (MPTP x± Mn) was as follows: SS, MPTP(-) x Mn(-); SLMn, MPTP(-) x low Mn(+); SHMn, MPTP(-) x high Mn(+); MpS, MPTP(+) x Mn(-); MpLMn, MPTP(+) x low Mn(+); MpHMn, MPTP(+) x high Mn(+). We administered MPTP (30 mg/kg per day) to male C57BL/6 mice intraperitoneally, once a day for 5 days. Subsequently, mice were treated with either 2 or 8 mg/kg of MnCl₂.4H₂O intraperitoneally, once a day for 3 weeks.

Blood and striatal Mn levels were elevated in the Mnexposed groups. The number of tyrosine hydroxylase (TH)-immunoreactive (ir) neurons in the substantia nigra pars compacta were decreased significantly in the MPTP-exposed groups. The densities of TH-ir axon terminals in caudate-putamen (CPU) were significantly decreased in the MPTP-treated groups. However, Mn treatment did not affect MPTP neurotoxicity. The densities of glial fibrillary acidic protein (GFAP)-ir astrocytes in the CPU or globus pallidus were significantly increased in the MPTP-treated groups. Concentrations of dopamine in the striatum were decreased significantly in the MPTP-exposed groups only, but Mn had no effect.

Key Words: manganese • MPTP • Parkinson disease • neurotoxicity • dopamine

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?