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Supplementation of ascorbic acid and -tocopherol prevents arsenic-induced protein oxidation and DNA damage induced by arsenic in rats

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India; Department of Radiology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA

K Sundaram

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India; Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA

S Mani

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India; Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA

S Samuel

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India

N Elango

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India

C Panneerselvam

Department of Medical Biochemistry, Dr. AL Mudhaliar PG Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600 113, India

Contamination of arsenic in drinking water is associated with several human diseases including cancer. It has been reported that oxidative stress plays a vital role in arsenic-induced biochemical and molecular alterations. The aim of the present study was to improve the understanding of arsenic-induced oxidative damage to proteins and to DNA and the role of antioxidants such as ascorbic acid and -tocopherol in alleviating arsenic-induced damages in experimental rats. A significant increase in the levels of protein oxidation, DNA strand breaks, and DNA–protein cross-links was observed in blood, liver, and kidney of rats exposed to arsenic (100 ppm in drinking water) for 30 days. Co-administration of ascorbic acid and -tocopherol to arsenic-exposed rats showed a substantial reduction in the levels of arsenic-induced oxidative products of protein and DNA. The results of this study support that free radical–mediated toxic manifestations of arsenic and also suggest that ascorbic acid and -tocopherol supplementation can improve the arsenic-induced molecular alterations.

Key Words: antioxidants • arsenic • DNA damage • oxidative stress • protein oxidation

Human & Experimental Toxicology, Vol. 26, No. 12, 939-946 (2007)
DOI: 10.1177/0960327107087909


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