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Effects of chlorinated biphenyls and metabolites on human uterine myocyte proliferation

Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden

Britt-Marie Bäcklin

Swedish Museum of Natural History, Contamination Research Group, Box 50007, SE-104 05 Stockholm, Sweden

Matts Olovsson

Department of Women's and Children's Health, Uppsala University, SE-751 85 Uppsala, Sweden, matts.olovsson{at}kbh.uu.se

Uterine myometrial cells are responsive to sex steroids, which could make them susceptible to actions of endocrine disrupting environmental contaminants such as some PCBs. The aim of this investigation was to identify possible effects of some chlorinated biphenyls (CBs) and their metabolites on myometrial cell proliferation. Myometrial cells obtained from women in both phases of the menstrual cycle and from pregnant women were grown in vitro and exposed to CB 101, CB 118, 3' -MeSO₂CB 101, 4'-MeSO₂-CB 101, 4-OH-CB 107, 17 ß-estradiol, progesterone, ethinylestradiol or levonorgestrel.

The proliferative activity was studied by a BrdU assay. Myometrial cell cultures originating from pregnant women exhibited decreased proliferation in response to 3'-MeSO ₂-CB 101, 4'-MeSO₂-CB 101 and 4-OH-CB 107. Estradiol, a combination of 1 nM 17ß-estradiol and 10 nM progesterone, ethinylestradiol and levonorgestrel also reduced the proliferation of the myometrial cells, regardless of whether the cells were collected from either of the menstrual cycle phases or from pregnant women. To our knowledge this study is the first to demonstrate that some CBs affect the proliferative activity of human uterine myocytes. Human & Experimental Toxicology (2007) 26, 801—809

Key Words: CB • in vitro • myometrial cells • PCB • proliferation • reproductive tissue

Human & Experimental Toxicology, Vol. 26, No. 10, 801-809 (2007)
DOI: 10.1177/0960327107084534


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