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Is there a role for PCR-SSCP among the methods for missense mutation detection of TP53 gene?

Department of Pharmacology and Toxicology, University of Oulu, Oulu, Finland

J A Welsh

Laboratory of Human Carcinogenesis, National Cancer Institute, NIH, Bethesda, MD 20892, USA

K H Vähäkangas

Department of Pharmacology and Toxicology, University of Kuopio, Kuopio, Finland, kirsi.vahakangas{at}uku.fi

Mutation analysis methods have increased in variety during the past years. High-throughput microarray methods have especially increased in popularity. However, new methods require reference points, and not all of the methods are equal in sensitivity and specificity. Furthermore, the detection of unknown missense mutations, such as unknown TP53 mutations in human tumors, for clinical purposes requires great accuracy, which may be difficult to acquire with the current high-throughput methods. For these reasons, the classical methods, such as PCR-manual sequencing and PCR-SSCP, are still valuable and necessary.

Key Words: comparison of methods • mutation analysis • optimisation • significance of TP53 mutations

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