Human & Experimental Toxicology

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here for more information

Sign In to gain access to subscriptions and/or personal tools.
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Angeli, J P.
Right arrow Articles by Mantovani, M S
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Angeli, J P.
Right arrow Articles by Mantovani, M S
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?
Human & Experimental Toxicology, Vol. 25, No. 6, 319-324 (2006)
DOI: 10.1191/0960327106ht631oa
© 2006 SAGE Publications

Anti-clastogenic effect of b-glucan extracted from barley towards chemically induced DNA damage in rodent cells

J Pf Angeli

Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil

L R Ribeiro

Departamento de Biologia Geral, UNESP, Rio Claro, SP, Brazil

M F Bellini

Departamento de Biologia Celular e Molecular, UNESP, Sã o José do Rio Preto, SP, Brazil

M S Mantovani

Departamento de Biologia Geral CCB, Universidade Estadual de Londrina Campus Universitário, P.O. Box 6001, CEP: 86051-990, Londrina, Paraná, Brazil; biomsm{at}uel.br

b-Glucan (BG) was tested in vitro to determine its potential clastogenic and/or anti-clastogenic activity, and attempts were made to elucidate its possible mechanism of action by using combinations with an inhibitor of DNA polymerase. The study was carried out on cells deficient (CHO-k1) and cells proficient (HTC) in phases I and II enzymes, and the DNA damage was assessed by the chromosomal aberration assay. BG did not show a clastogenic effect, but was anti-clastogenic in both cell lines used, and at all concentrations tested (2.5, 5 and 10 mg/mL) in combination with damage inducing agents (methylmethane sulfonate in cell line CHO-k1, and methylmethane sulfonate or 2-aminoanthracene in cell line HTC). BG also showed a protective effect in the presence of a DNA polymerase b inhibitor (cytosine arabinoside-3-phosphate, Ara-C), demonstrating that BG does not act through an anti-mutagenic mechanism of action involving DNA polymerase b.

Key Words: anti-mutagenesis • b-glucans • chromosomal aberrations • drug-metabolizing cells


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?