This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Galoch, A Articles by Kilanowicz, A Search for Related Content PubMed PubMed Citation Articles by Galoch, A Articles by Kilanowicz, A Social Bookmarking                   What's this?

Acute toxicity of polychlorinated naphthalenes and their effect on cytochrome P450

Department of Toxicology, Faculty of Pharmacy, Medical University, Lodz, Poland

A Sapota

Department of Toxicology, Faculty of Pharmacy, Medical University, Muszynskiego 1, 90-151 Lodz, Poland asapota{at}pharm.am.lodz.pl

M Skrzypinska-Gawrysiak

A Kilanowicz

Department of Toxicology, Faculty of Pharmacy, Medical University, Lodz, Poland

Polychlorinated naphthalenes (PCNs) are able to induce cytochrome P-450-dependent microsomal monooxygenase activities in vivo and in vitro. The aim of this study was to investigate the toxicity of a PCN mixture, and its effect on the levels of cytochrome P-450 in rats.

The animals were intragastrically administered a mixture of PCNs in single doses of 250, 500 and 1000 mg/kg b.w. Dissection of animals was performed 24, 72 and 240 hours after administration.

After PCN administration (all doses) the body weight loss (up to 30% in comparison with the control group, 240 hours after administration) and an increase of relative liver mass (about 126 - 153% of controls, 72 hours after administration) were observed.

The exposure to PCN evoked an increase in the level of total cytochrome P-450 as well as the activity of CYP 1A (mediated 7-ethoxyresorufin O-deethylation) at all time points. The maximum activity of CYP 1A (about 12-to 15-fold increase in comparison with the control group) was observed 72 hours after dosing.

Malondialdehyde (MDA), determined in the liver, showed a high increase and 240 hours after administration, the level of MDA was about one order of magnitude greater in comparison with control.

Key Words: cytochrome P-450 • polychlorinated naphthalenes • rats • toxicity

Human & Experimental Toxicology, Vol. 25, No. 2, 85-92 (2006)
DOI: 10.1191/0960327106ht574oa


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?