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Human & Experimental Toxicology, Vol. 25, No. 10, 599-604 (2006)
DOI: 10.1177/096032706072467

Free radical scavenging activities of yellow gentian (Gentiana lutea L.) measured by electron spin resonance

A Kussar

A Zupancic

Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, Ljubljana, Slovenia

M Sentjurc

J Stefan Institute, Jamova 39, Ljubljana, Slovenia

D Baricevicc

Department of Agronomy, Biotechnical Faculty, University of Ljubljana, Jamnikarjeva 101, SI-1000 Ljubljana, Slovenia dea.baricevic{at}bf.uni-lj.si

Yellow gentian (Gentiana lutea L.) is a herbal species with a long-term use in traditional medicine due to its digestive and stomachic properties. This paper presents an investigation of the free radical scavenging activity of methanolic extracts of yellow gentian leaves and roots in two different systems using electron spin resonance (ESR) spectrometry. Assays were based on the stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and the superoxide radicals (O2 +) generated by the xanthine/ xanthine oxidase (X/XO) system. The results of gentian methanolic extracts were compared with the antioxidant capacity of synthetic antioxidant butylated hydroxyanisole (BHA). This study proves that yellow gentian leaves and roots exhibit considerable antioxidant properties, expressed either by their capability to scavenge DPPH or superoxide radicals.

Key Words: antioxidants • electron spin resonance • Gentiana lutea L. • plant extracts • yellow gentian


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