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Colocalization of acetylcholinesterase, butyrylcholinesterase and choline acetyltransferase in rat spinal cord

Laboratory for Molecular Neurobiology, Institute of Pathophysiology, School of Medicine, University of Ljubljana, Zaloska 4, 1000 Ljubljana, Slovenia

In our previous in situ hybridization study in the adult rat spinal cord we demonstrated that only neurons express mRNAs for acetylcholinesterase (AChE; EC 3.1.1.7.) and butyrylcholinesterase (BuChE; EC 3.1.1.8.) in this CNS region; no staining was detected for these two messages in the glia cells. Here we used immunocytochemical staining for choline acetyltransferase (ChAT; EC 2.3.1.6.) to answer the, as yet only briefly approached, question whether the cells expressing the messages for both cholinesterases are cholinergic. All neurons expressing mRNAs for AChE and BuChE were found to be ChAT positive. This finding supports the previous proposal that BuChE modulates cholinergic transmission in AChE knockout mice.

Key Words: acetylcholinesterase mRNA • butyrylcholinesterase mRNA • choline acetyltransferase • cholinergic • spinal cord • ventral hor

Human & Experimental Toxicology, Vol. 24, No. 10, 543-545 (2005)
DOI: 10.1191/0960327105ht560oa


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