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Thioacetamide differentially affects the expression and activity of glutathione-S-transferase in the liver of Wistar rats

Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de Sao Paulo, Sáo Paulo, SP, Brazil

Beny Spira

Departamento de Microbiologia, Instituto de Ciencias Biomédicas, Universidade de Sáo Paulo, Av. Prof. Lineu Prestes, 1374 Sa benys{at}usp.br

Glutathione-S-transferase (GST) is a family of enzymes involved in the detoxification of toxic and carcinogenic compounds. In the present study, the effect of thioacetamide (TA), a hepatotoxic and hepatocarcinogenic compound, on the activity and expression of GST of Wistar female rats was tested. Animals were treated with a single dose of TA (250 mg/kg) for 12, 24, 48 and 72 hours. GST activity toward the broad substrate 1-chloro-2,4-dinitrobenzene was enhanced by TA. The protein level of the GST classes alpha and mu as well as the mRNA level of several GST subunits were also positively affected by the TA treatment. Female Wistar rats of the same age but from two other different colonies had their GST activity either inhibited or not affected by TA. The basal mRNA level of class alpha and class mu GST was also tested in female Wistar rats obtained from five different sources. Differences in the basal level of class alpha mRNA were observed in rats from at least three different sources, while class mu mRNA level was distinct in two groups of animals.

Key Words: gene expression • glutathione-S-transferase • rats • thioacetamide • Wistar

Human & Experimental Toxicology, Vol. 23, No. 9, 431-437 (2004)
DOI: 10.1191/0960327104ht469oa


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