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Cardiotoxicity in rats induced by methidathion and ameliorating effect of vitamins E and C

Suleyman Demirel University, School of Medicine, Department of Cardiovascular Surgery, Sevket Demirel Heart Center, Isparta, Turkey; turhan.kvc{at}doctor.com

Irfan Altuntas

Namik Delibas

Bekir Yildirim

Department of Biochemistry and Clinical Biochemistry, School of Medicine, Suleyman Demirel University, Isparta, Turkey

Ozden Candir

Department of Pathology, School of Medicine, Suleyman Demirel University, Isparta, Turkey

Ahmet Corâ

Department of Cardiovascular Surgery, School of Medicine, Suleyman Demirel University, Isparta, Turkey

Nermin Karahan

Department of Pathology, School of Medicine, Suleyman Demirel University, Isparta, Turkey

Erdogan Ibrisim

Ali Kutsal

Department of Cardiovascular Surgery, School of Medicine, Suleyman Demirel University, Isparta, Turkey

We have examined the effect of subchronic methidathion (MD) administration on heart damage, and have evaluated possible ameliorating effects of a combination of vitamins E and C against MD toxicity. The experimental groups were: control group, rats treated with 5 mg/kg MD and rats treated with 5 mg/kg body weight MD plus vitamin E and vitamin C (MD+Vit). The groups were given MD by gavage 5 days a week for four weeks at a dose level of 5 mg/kg/day (MD and MD+Vit) by using corn oil as the vehicle. Vitamin E and vitamin C were injected at doses of 50 mg/kg i.m. and 20 mg/kg i.p., respectively, after the treatment with MD in the MD+Vit group. The levels of malondialdehyde (MDA) were determined in the heart tissue, and the levels of cardiac troponin I (TnI) in serum. An autoanalyser was used to determine the serum activities of cholinesterase (ChE). Histopathological examination was carried out in the heart tissue. MDA significantly increased in the MD group as compared to controls (P <0.01). When MD was given concurrently with vitamins E and C, the increase in MDA was significantly less (P <0.01). ChE activity significantly decreased in the MD group as compared to controls (P <-0.01). When MD was given concurrently with vitamins E and C, the decrease in ChE activity was significantly higher (P <-0.05). The serum TnI levels significantly increased in the MD group as compared to controls (P <-0.01). When MD was given concurrently with vitamins E and C, the increase in the serum TnI was significantly less (P <-0.01). MD caused the diffuse loss of striation and myocytolysis of the cardiomyocytes, whereas the combination of vitamins E and C caused a significant decrease in these effects of MD. In conclusion, subchronic MD administration caused heart damage and, in addition, treatment with a combination of vitamins E and C after the administration of MD reduced heart damage caused by MD.

Key Words: cardiotoxicity • lipid peroxidation • methidathion • troponin I • vitamin C • vitamin E

Human & Experimental Toxicology, Vol. 23, No. 7, 323-329 (2004)
DOI: 10.1191/0960327104ht456oa


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