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Human & Experimental Toxicology
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Cytotoxicity of the phytosterol diosgenin and its derivatives in rat cultured hepatocytes and V79 fibroblasts

Patricia Silva Melo

Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), CP 6109, Campinas, SP, 13083-970, Brazil; pmelo{at}unicamp.br

Mariangela Burgos Martins De Azevedo

Scientia Tecnologia Química, Travessa R, 400, Cidade Universitária, São Paulo, SP, 05509-900, Brazil

Marco Antônio Teixeira Zullo

Laboratôrio de Fitoquímica, Instituto Agronômico, Caixa Postal 28, Campinas, SP, 13001-970, Brazil

João Batista Fabrin-Neto

Marcela Haun

Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, Campinas, SP, 13083-970, Brazil

In this work, the cytotoxic effects of some spirostane derivatives were examined in cultured hepatocytes and V79 fibroblasts using different viability assays. The derivatives were obtained by modifying the A and B rings of diosgenin. Diosgenin and its derivatives were more toxic in V79 fibroblasts (IC50 40-300 µM) than in hepatocytes (IC50 280-1000 µM). Inhibition of cytochrome P450IIIA in cultured hepatocytes by incubation with 1 mM cimetidine did not alter the toxicity of these compounds in these cells. These observations suggest that other pathways of detoxification may be involved in hepatocytes. In conclusion, the compounds studied merit investigation for their potential pharmacological and industrial applicability.

Key Words: cytotoxicity • diosgenin • hepatocytes • MTT • neutral red • saponin • steroidal sapogenin • V79 cells

Human & Experimental Toxicology, Vol. 23, No. 10, 487-493 (2004)
DOI: 10.1191/0960327104ht474oa
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