This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Kadikoylu, G Articles by Enli, Y Search for Related Content PubMed PubMed Citation Articles by Kadikoylu, G Articles by Enli, Y Social Bookmarking                         What's this?

The effects of desferrioxamine on cisplatininduced lipid peroxidation and the activities of antioxidant enzymes in rat kidneys

Adnan Menderes Universitesi Tip Fakultesi IRc Hastaliklari AD-Hematoloji BD-Aydin, Turkey gurhan{at}medscape.com

Z Bolaman

Adnan Menderes University, Medical Faculty, Division of Hematology, Aydin, Turkey

S Demir

Pamukkale University, Medical Faculty, Department of Biochemistry, Denizli, Turkey

M Balkaya

Adnan Menderes University, Faculty of Veterinary Medicine, Aydin, Turkey

N Akalin

Y Enli

Pamukkale University, Medical Faculty, Department of Biochemistry, Denizli, Turkey

Cisplatin-induced nephrotoxicity is associated with an increase in lipid peroxidation and oxygen free radicals in rat kidneys. In this study, the effects of desferrioxamine were compared to vitamin C and E on cisplatin-induced lipid peroxidation and antioxidant enzyme activities in rat kidneys. Rats were divided into five groups, with 15 Wistar rats in each group. In the control group, rats received 1 mL/100 g isotonic saline solution intraperitoneally (i.p.). In Group II, 10 mg/kg cisplatin i.p. was injected to rats. Thirty minutes before the same dosage of cisplatin administration, 100 mg/kg i.p. vitamin C or E was given to rats in groups III and IV, respectively. Rats in Group V received 250 mg/kg desferrioxamine i.p., before the same dose of cisplatin administration. All rats were killed by cervical dislocation after 72 hours. The kidneys were immediately removed and washed in cold saline. Spectrophotometric method was used for all analyses. While catalase, glutathione reductase (GR), and super oxide dismutase (SOD) levels were found to be significantly decreased (P B < 0.001), malondialdehyde (MDA) (P < 0.05) and hydrogen peroxide (H₂O₂) (P < 0.001) levels were significantly increased in the cisplatin group when compared to the controls. MDA levels were decreased by desferrioxamine (P < 0.005) as well as vitamin C and E (P < 0.05 and P < 0.001, respectively). These three compounds induced a significant increase in SOD levels (P B < 0.05), but only in the vitamin C group, were SOD levels not significantly different than the levels of the controls (P > 0.05). In the desferrioxamine (P < 0.05), vitamin C and E groups (P < 0.001 for both), the cisplatin elevated H₂O₂ levels were decreased. None of these drugs had any effect on GR and catalase levels (P > 0.05). Desferrioxamine is useful to prevent cisplatin-induced lipid peroxidation, however, vitamin C and E are more effective on antioxidant enzymes than desferrioxamine.

Key Words: antioxidant enzymes • cisplatin • desferrioxamine • lipid peroxidation • vitamin C • vitamin E

Human & Experimental Toxicology, Vol. 23, No. 1, 29-34 (2004)
DOI: 10.1191/0960327104ht413oa


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				F. Rombaldi, C. Cassini, M. Salvador, J. Saffi, and B. Erdtmann Occupational risk assessment of genotoxicity and oxidative stress in workers handling anti-neoplastic drugs during a working week Mutagenesis, March 1, 2009; 24(2): 143 - 148. [Abstract] [Full Text] [PDF]

				S. Lee, S.-O. Moon, W. Kim, M. J. Sung, D. H. Kim, K. P. Kang, Y. B. Jang, J. E. Lee, K. Y. Jang, S. Y. Lee, et al. Protective role of L-2-oxothiazolidine-4-carboxylic acid in cisplatin-induced renal injury Nephrol. Dial. Transplant., August 1, 2006; 21(8): 2085 - 2095. [Abstract] [Full Text] [PDF]