This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gumustekin, K. Articles by Gul, M. Search for Related Content PubMed PubMed Citation Articles by Gumustekin, K. Articles by Gul, M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Medline Plus Health Information Antioxidants Hazardous Substances DB MALONALDEHYDE NICOTINE NICOTINE TARTRATE Social Bookmarking                         What's this?

Vitamin E but not Hippophea rhamnoides L.prevented nicotine-induced oxidative stress in rat brain

Department of Physiology, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Konca Altinkaynak

Department of Biochemistry, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Handan Timur

Department of Physiology, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Seyithan Taysi

Department of Biochemistry, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Nuray Oztasan

Department of Physiology, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

M Fevzi Polat

Biotechnology Application and Research Center, Ataturk University 25240, Erzurum, Turkey

Fatih Akcay

Department of Biochemistry, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Halis Suleyman

Department of Pharmacology, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Senol Dane

Department of Physiology, Faculty of Medicine, Ataturk University 25240, Erzurum, Turkey

Mustafa Gul

Department of Physiology, Faculty of Medicine, Ataturk University, 25240, Erzurum, Turkeymgul{at}atauni.edu.tr

Oxidant effects of nicotine in the central nervous system is not clear. The aim of this study was to investigate whether nicotine induces oxidative stress in rat brain, and if it does, to test the effects of Hippophea rhamnoides L. extract (HRe-1) and also vitamin E as a positive control. The groups were: nicotine [0.5 mg/kg/day, intraperitoneal (i.p.)]; nicotine-vitamin E [75 mg/kg/day, intragastric (i.g.)]; nicotine-HRe-1 (250 mg/kg/day, i.g.); and control group (receiving only vehicles). There were eight rats per group and supplementation period was 3 weeks. Malondialdehyde (MDA) level was increased by nicotine in brain tissue, which was prevented by vitamin E whereas not affected by HRe-1. Brain tissue glutathione S-transferase activities of nicotine administered and HRe-1 supplemented groups were lower than control and vitamin E supplemented groups, while glutathione peroxidase (GSH-Px) activities of vitamin E and HRe-1 supplemented groups were lower than the nicotine administered group. Superoxide dismutase activity was not affected by any of the treatments. Total glutathione level was higher in the vitamin E supplemented group compared with control and nicotine administered groups. Vitamin E might have easily diffused to rat brain as a lipid soluble antioxidant, however, the plant extract, HRe-1, would not have sufficiently diffused to the brain to exert its antioxidant effect.

Key Words: brain • Hippophae rhamnoides L. • nicotine • oxidative stress • vitamin E

Human & Experimental Toxicology, Vol. 22, No. 8, 425-431 (2003)
DOI: 10.1191/0960327103ht383oa


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?