This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Jiann, B-P Articles by Jan, C-R Search for Related Content PubMed PubMed Citation Articles by Jiann, B-P Articles by Jan, C-R Social Bookmarking                         What's this?

Effect of triethyltin on Ca2+ movement in Madin–Darby canine kidney cells

Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, ROC

K-J Chou

Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, ROC

H-T Chang

Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, ROC

W-C Chen

Department of Surgery, Ping Tung Christian Hospital, Ping Tung 900, Taiwan, ROC

J-K Huang

Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan, ROC

C-R Jan

Department of Medical Education and Research, Kaohsiung Veterans General Hospital, 386 Ta Chung 1st Road, Kaohsiung 813, Taiwan, ROC; crjan{at}isca.vghks.gov.tw

The effects of the environmental toxicant, triethyltin, on Ca² + mobilization in Madin–Darby canine kidney (MDCK) cells have been examined. Triethyltin induced an increase in cytosolic free Ca² + levels ([Ca² +]_i) at concentrations larger than 2 mM in a concentrationdependent manner. Within 5 min, the [Ca² +]_i signal was composed of a gradual rise and a sustained phase. The [Ca² +]_i signal was partly reduced by removing extracellular Ca² +. In Ca² +-free medium, pretreatment with thapsigargin (1 mM), an endoplasmic reticulum Ca² + pump inhibitor, reduced 50 mM triethyltin-induced [Ca² +]_i increase by 80%. Conversely, pretreatment with triethyltin abolished thapsigargin-induced Ca² + release. Pretreatment with U73122 (2 mM) to inhibit phospholipase C-coupled inositol 1,4,5-trisphosphate formations failed to alter 50 mM triethyltin-induced Ca² + release. Incubation with triethyltin at a concentration (1 mM) that did not increase basal [Ca² +]_i for 3 min did not alter ATP (10 mM)and bradykinin (1 mM)-induced [Ca² +]_i increases. Collectively, this study shows that triethyltin altered Ca² + movement in renal tubular cells by releasing Ca² + from multiple stores in an inositol 1,4,5-trisphosphate-independent manner, and by inducing Ca² + influx.

Key Words: Ca2+ signaling • fura-2 • MDCK cells • thapsigargin • triethyltin

Human & Experimental Toxicology, Vol. 21, No. 8, 457-462 (2002)
DOI: 10.1191/0960327102ht276oa


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?