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The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidant enzymes in rat erythrocytes: role of vitamins E and C

Department of Biochemistry and Clinical Biochemistry, School of Medicine, Suleyman Demirel University, Isparta 32260, Turkey; irfanaltuntas{at}hotmail.com

N Delibas

R Sutcu

Department of Biochemistry and Clinical Biochemistry, School of Medicine, Suleyman Demirel University, Isparta 32260, Turkey

The effects of organophosphate insecticide methidathion (MD) on lipid peroxidation and anti-oxidant enzymes and the ameliorating effects of a combination of vitamins E and C against MD toxicity were evaluated in rat erythrocytes. Experimental groups were: control group, MD-treated group (MD), and MD+vitamin E+vitamin Ctreated group (MD+Vit). MD and MD+Vit groups were treated orally with a single dose of 8 mg/kg MD body weight at 0 hour. Vitamins E and C were injected at doses of 150 mg/kg body weight, i.m. and 200 mg/kg body weight, i.p., respectively, 30 min after the treatment of MD in the MD+Vit group. Blood samples were taken 24 hours after the MD administration. The level of malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) were studied in the erythrocytes. MDA level increased significantly in the MD group compared to the control group (P <0.05) and decreased significantly in the MD+Vit group compared to the MD group (P <0.05). The activities of SOD, GSH-Px, and CAT decreased in the MD group compared to the control group (P<0.05). Only GSH-Px activity increased in the MD+Vit group compared with the MD group. These results suggest that treating rats with MD increases LPO and decreases anti-oxidant enzyme activities in erythrocytes. Furthermore, single-dose treatment with a combination of vitamins E and C 30 min after the administration of MD can reduce LPO caused by MD.

Key Words: anti-oxidant enzymes • erythrocyte • lipid peroxidation • methidathion • vitamin C • vitamin E

Human & Experimental Toxicology, Vol. 21, No. 12, 681-685 (2002)
DOI: 10.1191/0960327102ht304oa


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