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Human & Experimental Toxicology
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*Compound via MeSH
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*METHANOL
*SODIUM METHOXIDE
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Alcohol and aldehyde dehydrogenase activity in the stomach and small intestine of rats poisoned with methanol

L Chrostek

D Szczepura

Department of Biochemical Diagnostics, Medical Academy, Bialystok, Poland

M Szmitkowski

Department of Biochemical Diagnostics, Medical Academy, Bialystok, Poland; Department of Biochemical Diagnostics, Medical Academy, Waszyngtona 15A, Bialystok, Poland

W Jelski

Department of Organic Chemistry, Pedagogical University, Siedlce, Poland

J Wierzchowski

Department of Biochemical Diagnostics, Medical Academy, Bialystok, Poland

The activities of alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH) were measured with fluorogenic naphthaldehydes in the stomach and small intestine homogenates of rats dosed with 6 g methanol/kg bw after 6, 12, 24 h and 2, 5, 7 days. After intoxication with a sublethal dose, the ADH activity measured with these naphthaldehydes andALDH activities in the stomach and small intestine were significantly decreased. This inhibition is stronger in the stomach and probably depends on cell damage and protein denaturation. We conclude that the activity measured with 6-methoxy-2-naphthaldehyde (MONAL-62) may be due to the activity of rat ADH-1 isoenzyme, and the activity detected with 4-methoxy-1-naphthaldehyde (MONAL-41) to the activity of rat ADH-2 isoenzyme.

Key Words: methanol intoxication • alcoholic enzymes

Human & Experimental Toxicology, Vol. 20, No. 5, 255-258 (2001)
DOI: 10.1191/096032701678227703
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