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Platelet dopamine receptors and oxidative stress parameters as markers of manganese toxicity

V K Khanna

Developmental Toxicology Division, Industrial Toxicology Research Centre, Post Box 80, MG Marg, Lucknow 226 001, India

A Roy

Department of Psychiatry, University of Illinois and West Side VA Medical Centre, Chicago, Illinois 1160612, USA

R Tandon

Developmental Toxicology Division, Industrial Toxicology Research Centre, Post Box 80, MG Marg, Lucknow 226 001, India

S Pradeep

Department of Molecular Biology, MGH, Boston, Massachusetts 002215, USA

P K Seth

Developmental Toxicology Division, Industrial Toxicology Research Centre, Post Box 80, MG Marg, Lucknow 226 001, India

The present study has been undertaken to investigate whether neurotoxic effects of manganese (Mn) are reflected in platelets in rats to monitor the usefulness of platelet as peripheral model. Exposure of rats to Mn (10 or 15 mg/kg bw, i.p.) for 45 days caused a significant increase in membrane fluidity as evidenced by decrease in fluorescence polarisation in platelets (11% and 14%) and striatum (9% and 13%). These rats exhibited a significant increase in superoxide dismutase activity both in platelets (24% and 37%) and striatum (31% and 42%), respectively, in comparison to controls. Exposure of rats to Mn for 45 days (15 mg/kg bw, i.p.) caused a significant decrease in reduced glutathione content (platelets 20%, striatum 24%) and catalase activity (platelets 35%, striatum 44%) compared to control rats. Rats exposed to Mn (10 or 15 mg/ kg bw, i.p.) for 15 days exhibited a significant increase in dopamine receptors both in platelets (55% and 40%) and striatum (38% and 31%). The results suggest that exposure to Mn may alter the membrane functions and impair the anti-oxidant defense mechanism both in platelets and brain. The study also suggests that dopaminergic mechanisms are impaired following Mn exposure and such changes are reflected in platelets. Interestingly, parallel changes both in striatum and platelets, as observed in the present study, strengthen the usefulness of platelets as a peripheral neuronal model.

Key Words: manganese • brain • platelets • dopamine receptors • oxidative stress

Human & Experimental Toxicology, Vol. 20, No. 12, 631-636 (2001)
DOI: 10.1191/096032701718890531


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