This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Durak, I Articles by Kagmaz, M Search for Related Content PubMed PubMed Citation Articles by Durak, I Articles by Kagmaz, M Social Bookmarking                         What's this?

Aspirin impairs antioxidant system and causes peroxidation in human erythrocytes and guinea pig myocardial tissue

Department of Biochemistry, Medical Faculty, Ankara University, Ankara; Turkey; Ankara Universitesi Tip Fakultesi, Biyokimya ABD (Dekanlik Binasi), Sihhiye, Ankara 06100, Turkey

M Karaayvaz

Department of Surgery, Medical Faculty, 100 Yil University, Van, Turkey

M Y B Çimen

A Avci

Department of Biochemistry, Medical Faculty, Ankara University, Ankara; Turkey

O B Çimen

Department of Physical Medicine and Rehabilitation, Ankara State Hospital, Ankara, Turkey

S Büyukkogak

H S Oztfirk

Department of Biochemistry, Medical Faculty, Ankara University, Ankara; Turkey

Hanefi Ozbek

Department of Surgery, Medical Faculty, 100 Yil University, Van, Turkey

M Kagmaz

Department of Biochemistry, Medical Faculty, Ankara University, Ankara; Turkey

This study aims to investigate possible effects of aspirin treatment on cellular oxidant/antioxidant system. In the first part of the study, 15 guinea pigs were given aspirin at three different doses (2200, 440 and 10 mg/kg/day) for 30 days and five were fed on the same diet without aspirin. After a month, animals were killed and their hearts were removed for use in analyses. In the other part, after fasting blood samples were obtained from 11 volunteer subjects, they were given aspirin (approximately 10 mg/kg/day) for 30 days and second blood samples were obtained after 1 month. Five volunteer subjects also participated as placebo control. Oxidant/antioxidant parameters, namely superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde (MDA), nonenzymatic superoxide scavenger activity (NSSA), susceptibility to oxidation (SO) and antioxidant potential (AOP) values, were assayed in the samples. Antioxidant system was found to be impaired in the heart tissue from guinea pigs and in the erythrocytes from volunteer subjects. AOP and NSSA values were lower and MDA higher after aspirin treatment in both heart tissues and erythrocytes. In guinea pig heart tissue, SO was lower, but GSH-Px and CAT were unchanged after aspirin treatment. In human erythrocytes, SO was unchanged, but GSH-Px and CAT activities were increased after aspirin treatment. Changes in guinea pig heart tissues from animals treated with higher aspirin doses were more drastic relative to those of human erythrocytes, but no meaningful differences were observed between analysis parameters of control and lower-dose (10 mg/kg/day) aspirin-treated animals. Our results suggest that high-dose aspirin exerts significant toxicity to guinea pig myocardium and normal dose aspirin may cause peroxidation in the human erythrocytes due to its oxidant potential. We suppose that antioxidant supplementation may be beneficial for the people using aspirin for longer periods in order to prevent peroxidation damages.

Key Words: aspirin • peroxidation • antioxidants • antioxidant system

Human & Experimental Toxicology, Vol. 20, No. 1, 34-37 (2001)
DOI: 10.1191/096032701674627721


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				K Dubey, D K Balani, and K K Pillai Potential adverse interaction between aspirin and Lisinopril in hypertensive rats Human and Experimental Toxicology, March 1, 2003; 22(3): 143 - 147. [Abstract] [PDF]