This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gyamfi, M. A. Articles by Aniya, Y. Search for Related Content PubMed PubMed Citation Articles by Gyamfi, M. A. Articles by Aniya, Y. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB AFLATOXIN B1 Social Bookmarking                         What's this?

Medicinal herb, Thonningia sanguinea protects against aflatoxin B1 acute hepatotoxicity in Fischer 344 rats

Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan

Yoko Aniya

Laboratory of Physiology and Pharmacology, School of Health Sciences, Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan

1. Thonningia sanguinea, a plant used prophylactically against bronchial asthma in Ghana was recently found to have antioxidative and hepatoprotective actions in our laboratory.

2. In this study, the effect of T. sanguinea extract on certain biochemical indices in serum and liver of Fischer 344 rats given a single intraperitoneal (i.p.) dose (1 mg/kg) of aflatoxin B1 (AFB1) was investigated.

3. Administration of AFB1 resulted in significant increases in serum alanine aminotransferase (ALT) and glutathione S-transferase (GST) levels and a signifi-cant decrease in aniline hydroxylase activity in liver microsomes. When T. sanguinea (5 ml/kg) was intraperitoneally administered to rats 12 h and 1 h before AFB1, liver injury was significantly reduced as seen in the decreased levels of serum ALT and serum GST. However, the decrease in aniline hydroxylase activity by AFB1 was not recovered but enhanced by T. sanguinea pre-treatment.

4. Kinetic analysis of cytochrome P450 activity of rat liver microsomes in vitro demonstrated that T. sanguinea inhibited aniline hydroxylase non-competitively suggesting depression of biotransformation of AFB1 to toxic metabolites.

5. The data indicate a hepatoprotective action of T. sanguinea against AFB1-induced liver injury.

Key Words: T. sanguinea • aflatoxin B1 • antioxidant • cytochrome P450 • hepatotoxicity • inhibition • monooxygenases

Human & Experimental Toxicology, Vol. 17, No. 8, 418-423 (1998)
DOI: 10.1177/096032719801700802


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				M A Gyamfi, N Hokama, K Oppong-Boachie, and Y Aniya Inhibitory effects of the medicinal herb, Thonningia san gunea, on liver drug metabolizing enzymes of rats Human and Experimental Toxicology, November 1, 2000; 19(11): 623 - 631. [Abstract] [PDF]