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Cytotoxicity of xenobiotics and expression of glutathione-S-transferases in immortalised rat hepatocyte cell lines

Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow G4 0NW

R Andrews

Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow G4 0NW

L Yin

Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow G4 0NW

R McLeod

Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY and Paisley, Paisley PA1 2BE, UK

C MacDonald

Biological Sciences, University of Paisley, Paisley PA1 2BE, UK

J D Hayes

Biomedical Research Centre, Ninewells Hospital and Medical School, University of Dundee, Dundee DD1 9SY

M H Grant

Bioengineering Unit, Strathclyde University, Wolfson Centre, Glasgow G4 0NW

1 Immortalised rat hepatocyte cell lines are more sensitive to the cytotoxicity of 1-chloro-2,4-dinitroben-zene and ethacrynic acid than primary cultures of hepatocytes.

2 Class alpha glutathione S-transferases are not expressed in immortalised hepatocyte cell lines. Class pi glutathione S-transferase expression is elevated in the immortalised cell lines compared with freshly isolated hepatocytes, but it is not as high as in the HTC rat hepatoma cell line.

3 Immortalised hepatocyte cell lines may provide a sensitive model system for detecting cytotoxicity associated with xenobiotics which are detoxified by glutathione S-transferases.

Key Words: immortalised hepatocytes • glutathione S-transferases • cytotoxicity

Human & Experimental Toxicology, Vol. 17, No. 3, 131-137 (1998)
DOI: 10.1177/096032719801700301


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