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Aspirin® and heparin prevent hepatic blood stasis and thrombosis induced by the toxic glycoprotein Bolesatine in mice

Université de Bordeaux II, Unité de Formation et de Recherche des Sciences Pharmaceutiques, 146, rue Léo Saignat 33076 Bordeaux

A Bingen

Unité de Recherches INSERM U 74 et Institut de Virologie de la FacultédeMédecinedel'Université Louis Pasteur, 67000 Strasbourg

E E Creppy

Université de Bordeaux II, Unité de Formation et de Recherche des Sciences Pharmaceutiques, 146, rue Léo Saignat 33076 Bordeaux

O Kretz

Université de Bordeaux II, Unité de Formation et de Recherche des Sciences Pharmaceutiques, 146, rue Léo Saignat 33076 Bordeaux

J P Gut

Unité de Recherches INSERM U 74 et Institut de Virologie de la FacultédeMédecinedel'Université Louis Pasteur, 67000 Strasbourg

L Dubuisson

Université de Bordeaux II, Laboratoire des Interactions cellulaires, 146, rue Léo Saignat 33076 Bordeaux, France

C Balabaud

Université de Bordeaux II, Laboratoire des Interactions cellulaires, 146, rue Léo Saignat 33076 Bordeaux, France

P Bioulac Sage

Université de Bordeaux II, Laboratoire des Interactions cellulaires, 146, rue Léo Saignat 33076 Bordeaux, France

A Kirn

Unité de Recherches INSERM U 74 et Institut de Virologie de la FacultédeMédecinedel'Université Louis Pasteur, 67000 Strasbourg

Bolesatine is a toxic glycoprotein isolated from Boletus satanas Lenz, which inhibits protein synthesis in vivo and in vitro. The LD50 (24 h) is 1 mg /kg bw (i.p.), in mice and rats. When given i.p. to mice (0.1 - 1.0 mg/kg bw) bolesatine induced thrombi and blood stasis in the liver, 5 - 21 h after injection, and modifications of the number of blood corpuscles in peripheral blood. These effects were efficiently reversed by aspirin, ticlopidin and heparin (as attested by histology and electron microscopy) which however failed to prevent death in animals given lethal doses. Together, these results showed that the death of bolesatine poisoned animals given high doses, was rather due to a combination of thrombosis and other toxic effects. In addition, they suggest that these antithrombotic drugs may overcome cases of human poisoning, with low exposures of this boletus, showing a hypertension probably due to mechanical obstruction which resists normal therapy.

Key Words: bolesatine • Boletus satanas • RBCs and platelets agglutination • mice • liver • thrombi • heparin • aspirin

Human & Experimental Toxicology, Vol. 17, No. 11, 620-624 (1998)
DOI: 10.1177/096032719801701106


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