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Metabolic capacities in cultured human hepatocytes obtained by a new isolating procedure from non-wedge small liver biopsies

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France

Catherine Viollon

Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, 4 Place Saint-Jacques, 25030 Besançon

Eliane Alexandre

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France

Agnés Azimzadeh

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France

Laurence Nicod

Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, 4 Place Saint-Jacques, 25030 Besançon

Philippe Wolf

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France, Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, 67098 Strasbourg, France

Daniel Jaeck

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France, Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, 67098 Strasbourg, France

Karim Boudjema

Fondation Transplantation, 5, avenue Moliére, 67200 Strasbourg, France, Centre de Chirurgie Viscérale et de Transplantation, Hôpital de Hautepierre, 67098 Strasbourg, France

Lysiane Richert

Laboratoire de Biologie Cellulaire, Faculté de Médecine et de Pharmacie, 4 Place Saint-Jacques, 25030 Besançon

A new isolating procedure of human hepatocytes has been developed using two-step collagenase digestion by a nonperfusion procedure (NP) of non-wedge liver biopsies.

1. A yield of 2 - 7610⁶ hepatocytes/g liver, 52 - 95% viability and 13 - 75% attachment were obtained from liver biopsies weighing 6 - 60 g, comparable to that obtained when using the classical perfusion procedure (P) to isolate human hepatocytes from wedge liver samples of 50 - 150 g.

2. In culture, human hepatocytes obtained by NP remained attached to plastic for up to 5 days and displayed the usual morphological characteristics.

Their metabolic capacities, assessed by liver-specific albumin and urea synthesis and by CYP-dependent and conjugation pathways, were equivalent to those of human hepatocytes obtained by P. In addition, they responded adequately to specific CYP inducers, demonstrating that they constitute a model in which human drug metabolism and toxicity studies can be performed.

Key Words: human hepatocyte isolation • metabolic capacities • CYP isoforms • inducers

Human & Experimental Toxicology, Vol. 17, No. 10, 544-553 (1998)
DOI: 10.1177/096032719801701004


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