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SECMA 1®, a mitogenic hexapeptide from Ulva algeae modulates the production of proteoglycans and glycosaminoglycans in human foreskin fibroblast

Palmer research, 18, rue de Coulon, 33640, Arbanats, Laboratory of Toxicology and Applied Hygiene, 146, rue Léo Saignat, University Victor Segalen Bordeaux 2, 33076, Bordeaux, France; 22260, France

D Saboureau

Palmer research, 18, rue de Coulon, 33640, Arbanats

N Mekideche

SECMA, marin biotechnology, Pontrieux

E E Creppy

Laboratory of Toxicology and Applied Hygiene, 146, rue Léo Saignat, University Victor Segalen Bordeaux 2, 33076, Bordeaux, France; 22260, France

SECMA 1® is a polypeptide purified from a green algeae of the Ulva species by several gel chromatographies, showing the following sequence (Glu-Asp-Arg-Leu-Lys-Pro). In order to determine the effect of SECMA 1® on human skin fibroblasts extracellular matrix, proteoglycans (PGs) and glycosaminoglycans (GAGs) were assayed after 24 h incubation of 20 day-old foreskin fibroblasts at the 2nd passage.

The results revealed that most of [³⁵S]sulphate was associated with fibroblast membranes, which contained (67%) of the total de novo synthesized sulphated PGs, in two distinct forms: one hydrophilic (39%), and one hydrophobic (28%). The remaining `matrix' retained 5% of proteoglycans. The remaining ³⁵S-label may represent the free label in the cytosol.

After 24 h incubation of skin fibroblasts with different concentrations of SECMA 1® (2, 4 and 10 µg/ml), the [³⁵S] sulphate incorporation into PGs of Salt-extract, sodium deoxycholate (DOC) extract and Guanidine hydrochloride (GuA-HCl)-extract was increased significantly (P<0.005) with 4 µg/ml, as compared to untreated control. The most effective concentration (4 µg/ml) increased the different [³⁵S]sulphate PGs extracts (NaCl, DOC and GuA-HCl) by respectively (66; 17 and 75%).

The relative contents of iduronic and glucuronic acid in the GAG produced by skin fibroblasts were estimated. No effect of SECMA 1® on the incorporation of [³⁵S]sulphate into Heparan sulphate was found. The incorporation of [³⁵S]sulphate into (chondroïtine sulphate + heparan sulphate) and (chondroïtine sulphate + dermatan sulphate) was increased by respectively 37% and 11% by SECMA 1® (4 µg/ml).

Key Words: SECMA 1® hexapeptide • human skin fibroblasts • DNA synthesis • [35S]incorporation • proteoglycans • glycosaminoglycans

Human & Experimental Toxicology, Vol. 17, No. 1, 18-22 (1998)
DOI: 10.1177/096032719801700103


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