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Kinetic analysis of the intestinal iron absorption process in situ. The potential of vascularly autoperfused intestinal loops

Walther-Straub-Institut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universtitdt, Nufßbaumstr. 26, 80336 München

K. Osterloh

Walther-Straub-Institut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universtitdt, Nufßbaumstr. 26, 80336 München

K. Schümann

Walther-Straub-Institut für Pharmakologie und Toxikologie der Ludwig-Maximilians-Universtitdt, Nufßbaumstr. 26, 80336 München

1 Blood sampling from mesenteric venules during absorption in situ is a useful tool to analyse intestinal absorption kinetics and prehepatic metabolism in different sections of the rat small intestine. By use of a micromanipulator, the method can be applied to the duodenum. This part of the small intestine shows the strongest adaptation of non-haem iron absorption to the demand for iron.

2 Iron absorption kinetics was linear in duodenal and jejunal segments. In iron-deficient animals, intestinal iron absorption capacity was increased in the duode num, while simultaneously determined galactose absorption showed no change.

3 In situ perfusion and cannulation of mesenteric venules in duodenal segments are described. The use of a micromanipulator permits varying the blood volume collected by changing the vertical angle between the cannula and the mesenteric vessel.

4 Intestinal iron absorption rates remained close to constant when blood flow rates were varied by a factor of about ten. Plasma concentrations of absorbed iron vs mesenteric blood flow rates followed a hyperbolic function, as the plasma concentration of absorbed iron in mesenteric venules increased to the same extent as the blood flow decreased.

5 As the plasma transferrin concentration did not change over the experimental period, the concentra tion of absorbed iron in the mesenteric plasma exceeded the iron-binding capacity of plasma trans ferrin at low blood flow rates. This observation shows that enhancement of intestinal iron absorption does not require a corresponding increase in plasma iron- binding capacity in the intestinal tissue.

6 Vascularly perfused gut loops were also used to measure prehepatic metabolism, which may influence organotropism of carcinogenic metabolites. Therefore, this type of preparation is likely to find a variety of toxicological applications.

Key Words: iron • absorption • transferrin • in situ perfusion • blood flow rate

Human & Experimental Toxicology, Vol. 16, No. 8, 425-428 (1997)
DOI: 10.1177/096032719701600802


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