This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Clarke, H. Articles by Ryan, M. Search for Related Content PubMed PubMed Citation Articles by Clarke, H. Articles by Ryan, M. Social Bookmarking                   What's this?

-glutathione s-transferase (-GST) release, an early indicator of carbon tetrachloride hepatotoxicity in the rat

Department of Pharmacology, University College Dublin, Fosters Avenue, Blackrock, Dublin 4, Ireland

DA Egan

Department of Pharmacology, University College Dublin, Fosters Avenue, Blackrock, Dublin 4, Ireland

M. Heffernan

BIOTRIN International, 93 The Rise Mount Merrion, Co. Dublin, Ireland

S. Doyle

BIOTRIN International, 93 The Rise Mount Merrion, Co. Dublin, Ireland

C. Byrne

BIOTRIN International, 93 The Rise Mount Merrion, Co. Dublin, Ireland

C. Kilty

BIOTRIN International, 93 The Rise Mount Merrion, Co. Dublin, Ireland

MP Ryan

Department of Pharmacology, University College Dublin, Fosters Avenue, Blackrock, Dublin 4, Ireland

1 The use of the cytoplasmic enzyme, alpha glutathione s-transferase (-GST) as an early index of carbon tetrachloride (CCl₄) toxicity in the rat was investigated and compared with a standard enzyme marker, aspartate aminotransferase (AST). The hepatotoxic effects of CCl ₄ in the rat were determined in a time and dose-response study.

2 Following CCl ₄ exposure, -GST release was shown to be an earlier and more sensitive biomarker of hepatotoxicity than AST.

3 Significant increases in -GST were detected 2 h after CCl₄ exposure. Using the enzyme marker AST, this early hepatotoxic injury went undetected. At 6 and 16 h, -GST was also a more sensitive indicator of hepatotoxicity than AST.

4 -GST release was significantly increased at a dose of 5 µl/kg, the lowest concentration of CCl₄ administered and clearly responded in a dose-dependent manner with increasing doses of CCl₄. In contrast, release of AST did not reach statistical significance until a dose of 25 µl/kg.

5 Thus, these findings indicate that -GST is a more sensitive and more accurate reflector of CCl₄ induced hepatotoxicity than AST.

Key Words: glutathione s-transferase • -GST • aspartate amino transferase • AST • hepatotoxicity • carbon tetrachloride • biomarker

Human & Experimental Toxicology, Vol. 16, No. 3, 154-157 (1997)
DOI: 10.1177/096032719701600304


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				V. Tong, X. W. Teng, T. K. H. Chang, and F. S. Abbott Valproic Acid I: Time Course of Lipid Peroxidation Biomarkers, Liver Toxicity, and Valproic Acid Metabolite Levels in Rats Toxicol. Sci., August 1, 2005; 86(2): 427 - 435. [Abstract] [Full Text] [PDF]

				A. Serafin, J. Rosello-Catafau, N. Prats, C. Xaus, E. Gelpi, and C. Peralta Ischemic Preconditioning Increases the Tolerance of Fatty Liver to Hepatic Ischemia-Reperfusion Injury in the Rat Am. J. Pathol., August 1, 2002; 161(2): 587 - 601. [Abstract] [Full Text] [PDF]

				D E Amacher A toxicologist's guide to biomarkers of hepatic response Human and Experimental Toxicology, May 1, 2002; 21(5): 253 - 262. [Abstract] [PDF]

				P. S. Giffen, C. R. Pick, M. A. Price, A. Williams, and M. J. York Alpha-Glutathione S-Transferase in the Assessment of Hepatotoxicity--Its Diagnostic Utility in Comparison with Other Recognized Markers in the Wistar Han Rat Toxicol Pathol, April 1, 2002; 30(3): 365 - 372. [Abstract] [PDF]

				D. J. Koo, M. Zhou, I. H. Chaudry, and P. Wang Plasma {alpha}-Glutathione S-Transferase: A Sensitive Indicator of Hepatocellular Damage During Polymicrobial Sepsis Arch Surg, February 1, 2000; 135(2): 198 - 203. [Abstract] [Full Text] [PDF]