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Membrane attack induced by HlyA, a pore-forming toxin of Vibrio cholerae

University Laboratory of Physiology, Parks Road, Oxford OX1 3PT, England, UK

Venugopal Sathyamoorthy

Division of Virulence Assessment, Centre for Food Safety and Applied Nutrition, Food and Drug Administration, 200 'C' Street, SW, Washington DC 20204, USA

Robert H Hall

Division of Virulence Assessment, Centre for Food Safety and Applied Nutrition, Food and Drug Administration, 200 'C' Street, SW, Washington DC 20204, USA

Andrew C Hall

Department of Physiology, University Medical School, Teviot Place, Edinburgh EH8 9AG, Scotland, UK

Determining the activity of purified toxins has generally provided the basis for establishing their role in the host- pathogen relationship. The bacterial genus Vibrio pro duces a number of exotoxins in addition to cholera toxin, including haemolysin A (HIyA; Vibrio cholerae) and thermostable direct haemolysin (TDH; Vibrio parahae molyticus), both of which possess membrane-targeting cytolytic activity. The action of HlyA has been analyzed using protocols previously applied to TDH: lysis and flux experiments on human erythrocytes showed that HlyA similarly causes lysis after cell swelling (by colloid osmosis) due to an elevation of cation permeability. However, kinetic measurements of flux, haemolysis and cation selectivity showed that HlyA and TDH form pores with distinct and characteristic features.

Key Words: HlyA • Vibrio cholerae • El Tor • bacterial toxin • haemolysis • red blood cell

Human & Experimental Toxicology, Vol. 16, No. 2, 101-105 (1997)
DOI: 10.1177/096032719701600205


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