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Comparison of toxicity induced by HT-2 toxin on human and rat granulo-monocytic progenitors with an in vitro model

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, ISAMOR, Technopôle Brest-Iroise, 29280 Plouzané, France

D. Parent-Massin

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, ISAMOR, Technopôle Brest-Iroise, 29280 Plouzané, France

B. Rio

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, ISAMOR, Technopôle Brest-Iroise, 29280 Plouzané, France

H. Hoellinger

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire de Brest, ISAMOR, Technopôle Brest-Iroise, 29280 Plouzané, France

HT-2 toxin is a trichothecene mycotoxin occuring naturally in various agricultural products. Many in vitro studies have shown that HT-2 toxin is a major metabolite of the parent compound T-2 toxin. In man as well as in animals both toxins have been shown to cause alimentary intoxications and haematological disorders. Granulo- monocytic progenitors (CFU-GM) from human umbilical cord blood and from rat bone marrow were cultured in the presence of HT-2 toxin (10^-7 M to 10^-10 M) for 14 days. The concentration-effect relationship was studied and the IC ₅₀ were determined on Days 7, 10 and 14. The IC₅₀ was 1.8 x 10^-9 M, 3.5 x 10^-9 M and 1.8 x 10^-9 M for hu man cells, and 2 x 10 ^-9 M, 2.3 x 10^-9 M and 2.2 x 10^-9 M for rat cells. The results have been compared with previous findings for T-2 toxin. Although T-2 and HT-2 toxins had a similar IC₅₀ on human and rat CFU-GM, the expression of their cytotoxicities was different. These findings are relevant to investigation of the cellular targets of T-2 and HT-2 in elucidating the mechanism of trichothecene haematotoxicity.

Key Words: HT-2 • mycotoxin • granulo-monocytic progenitors • man • rat

Human & Experimental Toxicology, Vol. 15, No. 3, 208-213 (1996)
DOI: 10.1177/096032719601500304


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