This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Rompelberg, C. Articles by Verhagen, H. Search for Related Content PubMed PubMed Citation Articles by Rompelberg, C. Articles by Verhagen, H. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB ACETAMINOPHEN EUGENOL MITOMYCIN C VINBLASTINE Social Bookmarking                         What's this?

Effect of short-term dietary administration of eugenol in humans

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

Jtwe Vogels

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

N. de Vogel

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

Gcdm Bruijntjes-Rozier

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

WH Stenhuis

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

Jjp Bogaards

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

H. Verhagen

TNO Nutrition and Food Research Institute, PO Box 360, 3700 AJ Zeist, The Netherlands

1 In order to study the antigenotoxic potential of eugenol in humans, ten healthy non-smoking males ingested a daily amount of 150 mg eugenol or the placebo for seven consecutive days. After a washout period of one week, groups ingesting eugenol or the placebo were crossed and received the other treatment for seven consecutive days.

2 On days 8 and 22 blood samples were taken for the assessment of standard clinical biochemical parameters. To study the possible antigenotoxic effect of eugenol, on day 8 and 22 blood samples were collected and exposed in vitro to the established genotoxic agents mitomycin C and vinblastine. After exposure the percentage of cells with chromosome aberrations and micronuclei was deter mined in cultured white blood cells. On days 8 and 22 paracetamol (500 mg p.o.) was administered as test substance to measure phase-II biotransformation capa city. Glutathione-S-transferase (GST) activities were determined in erythrocytes and blood plasma.

3 No significant differences in the clinical biochemical parameters were detected between the eugenol-period and the placebo-period, indicating that daily administration of 150 mg eugenol for 7 days has no toxic affects.

4 No significant differences on the cytogenetic para meters were found after ingestion of eugenol. Thus, there are no indications for an antigenotoxic potential of eugenol in humans, consuming daily 150 mg eugenol for 7 days.

5 A significant reduction in -class GSTs in plasma (P<0.05), but not in the other measured biotransformation parameters, was found in volunteers during the eugenol- period as compared to the placebo-period. This may either reflect GST-inhibition by eugenol or protection against background damage of liver cells by eugenol.

Key Words: eugenol • antigenotoxicity • GST

Human & Experimental Toxicology, Vol. 15, No. 2, 129-135 (1996)
DOI: 10.1177/096032719601500205


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. W Lampe Spicing up a vegetarian diet: chemopreventive effects of phytochemicals Am. J. Clinical Nutrition, September 1, 2003; 78(3): 579S - 583. [Abstract] [Full Text] [PDF]