This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Gachet, C. Articles by Cazenave, J. Search for Related Content PubMed PubMed Citation Articles by Gachet, C. Articles by Cazenave, J. Social Bookmarking                         What's this?

Bolesatine induces agglutination of rat platelets and human erythrocytes and platelets in vitro

INSERM U.311, Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, 10 rue Spielmann, 67085 Strasbourg CEDEX

R. Ennamany

Laboratoire de Toxicologie et d'Hygiène Appliquée, Université de Bordeaux 2, 3ter place de la Victoire, 33000 Bordeaux

O. Kretz

Laboratoire de Toxicologie et d'Hygiène Appliquée, Université de Bordeaux 2, 3ter place de la Victoire, 33000 Bordeaux

P. Ohlmann

INSERM U.311, Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, 10 rue Spielmann, 67085 Strasbourg CEDEX

C. Krause

INSERM U.311, Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, 10 rue Spielmann, 67085 Strasbourg CEDEX

EE Creppy

Laboratoire de Toxicologie et d'Hygiène Appliquée, Université de Bordeaux 2, 3ter place de la Victoire, 33000 Bordeaux

G. Dirheimer

Institut de Biologie Moléculaire et Cellulaire du CNRS and Université Louis Pasteur, 15 rue Descartes, 67084 Strasbourg CEDEX, France

JP Cazenave

INSERM U.311, Biologie et Pharmacologie des Interactions du Sang avec les Vaisseaux et les Biomatériaux, Centre Régional de Transfusion Sanguine, 10 rue Spielmann, 67085 Strasbourg CEDEX

Bolesatine is a toxic glycoprotein isolated from the mushroom Boletus satanas Lenz, which has been shown to inhibit protein synthesis in cell-free systems and cell culture. It is toxic to rodents, the LD₅₀% 24 h being 1 mg kg^-1 (i.p.) and 0.15 mg kg^-1 (i.v) in the rat in which it induces hepatic blood stasis.

Bolesatine possesses lectinic properties with in parti cular a sugar binding site for D-galactose and mitogenic activity toward lymphocytes.

Tested for cell agglutination on red blood cells and platelets, bolesatine agglutinates both human and rat platelets from threshold concentrations of 30 and 300 nM respectively.

EDTA and PGI₂ (aggregation inhibitors) do not decrease the agglutination induced by bolesatine, indicating that the process does not involve platelet activation. In contrast, fibrinogen decreases platelet agglutination in duced by bolesatine, most likely by masking the binding sites on platelets or by interacting with the toxin.

Bolesatine agglutinates all red blood cells without any blood group specificity in the concentration range of 20 to 40 nM. This haemagglutination cannot be prevented by sugars, including D-galactose at a concentration of 0.5 M.

Key Words: Bolesatine • Boletus satanas • agglutination • aggrega tion • rat • man

Human & Experimental Toxicology, Vol. 15, No. 1, 26-29 (1996)
DOI: 10.1177/096032719601500105


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?