This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Astier, A. Articles by Baud, F. J Search for Related Content PubMed PubMed Citation Articles by Astier, A. Articles by Baud, F. J Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB CYANOCOBALAMIN HYDROXOCOBALAMIN Social Bookmarking                         What's this?

Complexation of intracellular cyanide by hydroxocobalamin using a human cellular model

Laboratoire de Toxicologie, Service de Pharmacie, CHU Henri Mondor 94010 Créteil, France

Frederic J Baud

Réanimation Toxicologique, Hopital Fernand Widal, Paris, France

1 The rational for administering hydroxocobalamin (OHCbl) as an antidote to cyanide poisoning is based on the high affinity ofCN ion for cobalt compounds. However, only few data are available on the influence of OHCbl on the intracellular cyanide pool.

2 In human fibroblasts incubated for 10 min with 500 µM of [¹⁴C] cyanide, the accumulation ratio was 25 at 37°C (10.45 ± 1.51 mM) and 11.9 at 4°C.

3 Using the monoblastic U-937 cell line, a rapid uptake of radioactive cyanide was observed with a maximum accumulation ratio of 1.97 at 5 min.

4 A linear relationship between cyanide uptake by U-937 cells and cyanide concentration in incubation medium (10-500 µM; 5 min) was found suggesting a first order process (k= 0.25 min^-1).

5 After incubation of fibroblasts with 500 µM of OHCbl, a 75% decrease of intracellular cyanide was observed, with concomittant formation of intracellular cyanocobalamin CNCbl (intracellular/extracellular ratio: 158).

6 These findings suggest that OHCbl is able to penetrate into heavily cyanide loaded cells and to complex cyanide to the non-toxic CNCbl form.

Key Words: cyanide • antidote • hydroxocobalamin • cyanocobala min • cellular model

Human & Experimental Toxicology, Vol. 15, No. 1, 19-25 (1996)
DOI: 10.1177/096032719601500104


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?