This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Lautraite, S. Articles by Hoellinger, H. Search for Related Content PubMed PubMed Citation Articles by Lautraite, S. Articles by Hoellinger, H. Social Bookmarking                         What's this?

Comparison of toxicity induced by T-2 toxin on human and rat granulo-monocytic progenitors with an in vitro model

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire, Isamor, Technopôle Brest-Iroise, 29280, Plouzané, France

D. Parent-Massin

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire, Isamor, Technopôle Brest-Iroise, 29280, Plouzané, France

B. Rio

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire, Isamor, Technopôle Brest-Iroise, 29280, Plouzané, France

H. Hoellinger

Laboratoire de Microbiologie et de Biochimie, Ecole Supérieure de Microbiologie et Sécurité Alimentaire, Isamor, Technopôle Brest-Iroise, 29280, Plouzané, France

T-2 toxin is a trichothecene mycotoxin produced by vari ous species of fungi. Trichothecenes are known as major contaminants of cereals and their derivatives. In man as well as in animals, T-2 toxin has been shown to induce ali mentary intoxication and, among others, haematological symptoms. Granulo-monocytic progenitors from human umbilical cord blood on the one hand and granulo-mono cytic progenitors from rat bone marrow on the other, were cultured in the presence of T-2 toxin (from 10^-7 to 10^-10 M) for 14 days. A study of concentration and effect relation ships showed a strong and rapid effect of T-2 toxin on rat colony forming unit-granulocyte and macrophage (CFU-GM) between 5.10^-9 M and 10^-9 M. On the other hand, human CFU-GM were able to grow in the presence of the same T-2 toxin concentrations. IC₅₀ were determined on day 7, 10 and 14. They were, respectively, 1.6.10-9 M; 3.6.10^-9 M; 1.4.10^-9 M for human cells, and 2.2.10^-9 M; 3.3.10^-9 M; 2.6.10 ^-9 M for rat cells. The present study was prompted by the need to define precisely the cytotoxic and inhibitory T-2 toxin concentrations for rat and human CFU-GM. It is particularly relevant for the investigation of cellular T-2 toxin targets and in order to elucidate the mechanism of trichothecene haematotoxicity.

Key Words: T-2 toxin • mycotoxin • granulo-monocytic progenitors • man • rat

Human & Experimental Toxicology, Vol. 14, No. 8, 672-678 (1995)
DOI: 10.1177/096032719501400808


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				R Froquet, G Le Drean, and D Parent-Massin Effect of Ochratoxin a on human haematopoietic progenitors proliferation and differentiation: an in vitro study Human and Experimental Toxicology, July 1, 2003; 22(7): 393 - 400. [Abstract] [PDF]

				R Froquet, Y Sibiril, and D Parent-Massin Trichothecene toxicity on human megakaryocyte progenitors (CFU-MK) Human and Experimental Toxicology, February 1, 2001; 20(2): 84 - 89. [Abstract] [PDF]

				B. Rio, S. Lautraite, and D. Parent-Massin In vitro toxicity of trichothecenes on human erythroblastic progenitors Human and Experimental Toxicology, November 1, 1997; 16(11): 673 - 679. [Abstract] [PDF]

				S. Lautraite, D. Parent-Massin, B. Rio, and H. Hoellinger Comparison of toxicity induced by HT-2 toxin on human and rat granulo-monocytic progenitors with an in vitro model Human and Experimental Toxicology, March 1, 1996; 15(3): 208 - 213. [Abstract] [PDF]