This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Arroyo, C. M Articles by Broomfield, C. A Search for Related Content PubMed PubMed Citation Articles by Arroyo, C. M Articles by Broomfield, C. A Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 2-CHLOROETHYL ETHYL SULFIDE BIS(2-CHLOROETHYL)SULFIDE Social Bookmarking                         What's this?

Activation of alpha-human tumour necrosis factor (TNF-) by human monocytes (THP-1) exposed to 2-chloroethyl ethyl sulphide (H-MG)

US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland USA

Robert L Von Tersch

US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland USA

Clarence A Broomfield

US Army Medical Research Institute of Chemical Defense, Aberdeen Proving Ground, Maryland USA

Tumour necrosis factor (TNF) is a monokine produced by monocytes and macrophages in response to different stim uli. To determine whether vesicant agents such as half- mustard gas (H-MG; chemical structure: ClCH₂CH ₂SCH₂CH₃) may induce the release of TNF- in human monocytes (THP-1), ELISA experiments were con ducted at different post exposure times. The results indi cate that: (1) Significant increases in the TNF- (pg mL^-1) concentration were observed as a function of time when THP-1 cells were exposed to 100 µL of 2 M H-MG. A spe cific serine-type protease inhibitor, N-p-tosyl-L-lysine chloromethyl ketone (TLCK), led to partial but significant inhibition of TNF activation. (2) Furthermore, this labora tory detected the generation of spin adducts of 2-methyl-2- nitrosopropane (MNP) having a resemblance to MNP adducts generated from hydrogen atom abstraction of pro tein constituents.¹ The EPR/Spin Trapping data indicate the trapping of by-products of protein degradation after exposure to H-MG. TNF- may play a role as a biochemi cal marker for pathophysiological changes induced by H- MG or related agents.

Key Words: TNF- • human monocyte • THP-1 • ELISA • EPR/Spin trapping • MNP • protein degradation • half-mus tard gas • vesicant agent

Human & Experimental Toxicology, Vol. 14, No. 7, 547-553 (1995)
DOI: 10.1177/096032719501400701


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				C. M. Arroyo, C. A. Broomfield, and B. E. Hackley Jr. The Role of Interleukin-6 (IL-6) in Human Sulfur Mustard (HD) Toxicology International Journal of Toxicology, September 1, 2001; 20(5): 281 - 296. [Abstract] [PDF]