This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Steffen, V. Articles by Cano, J. Search for Related Content PubMed PubMed Citation Articles by Steffen, V. Articles by Cano, J. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB 1-METHYL-4-PHENYL-1,2,3,6-TETRAHYDROPYRIDINE Social Bookmarking                         What's this?

Effect of intraventricular injection of 1-methyl-4-phenylpyridinium: protection by acetyl-L-carnitine

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

M. Santiago

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

CP de la Cruz

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

E. Revilla

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

A. Machado

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

J. Cano

Departamento de Bioquímica, Bromatologia y Toxicologia, Facultad de Farmacia, Universidad de Sevilla, C/ Prof García Gonzalez, Sevilla 41012, Spain

1-methyl-4-phenylpyridinium (MPP+) is the bioactivated product of 1-methyl-4-phenyl- 1, 2, 3, 6-tetrahydropyri dine (MPTP). The neurotoxic action of MPP+ injected intracerebroventricularly (ICV) in the rat has been stud ied, using dopaminergic systems in the substantia nigra, striatum, olfactory bulb, median eminence and hypoph ysis. The following results were obtained: (1) Rats with ICV administration of 1 µl MPP+ solution (62.5 µg of MPP+ rat) showed 50% mortality; (2) The ICV administration of MPP+ produced a decrease in dopamine (DA) concentra tion in different areas of rat CNS studied: striatum (83%), hypophysis (95%) and median eminence (70%). However, olfactory bulb and substantia nigra were not affected; (3) MPP+ by ICV administration produced neurotoxic effect on the dopaminergic system. We also studied the possible protective action of acetyl-L-carnitine (ALC) against the neurotoxic action of MPP+. Rats were intraperitoneally injected daily for 8 days with 100 mg kg^-1 of ALC and 3 days from the beginning of the MPP+ treatment; (4) We found that the ALC treatment significantly protected against mortality produced by the ICV injection of MPP⁺. Rats treated with ALC showed no mortality; (5) We did not find a protective effect on the dopaminergic system study ing either catecholamine concentration or measuring tyro sine hydroxylase, neurofilament or glial fibrillary acid protein; (6) The results suggest that the ALC protective action could be related to energy metabolism.

Key Words: acetyl-L-carnitine • 1-methyl-4-phenylpyri dinium • protection • striatum

Human & Experimental Toxicology, Vol. 14, No. 11, 865-871 (1995)
DOI: 10.1177/096032719501401102


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?