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Aluminium deposition in liver and kidney following acute intravenous administration of aluminium chloride or citrate in conscious rats

Department of Physiology, University of Birmingham, Birmingham, B15 2TT, UK

JA Wood

Department of Physiology, University of Birmingham, Birmingham, B15 2TT, UK

HC Saunders

Department of Physiology, University of Birmingham, Birmingham, B15 2TT, UK

MS Freeman

Department of Physiology, University of Birmingham, Birmingham, B15 2TT, UK

CJ Lote

Department of Physiology, University of Birmingham, Birmingham, B15 2TT, UK

1 Plasma, urinary, liver and kidney cell aluminium (Al) levels were monitored in the rat, 1h after intravenous administration of 29630 nmol (800 µg) Al as either Al chloride or as Al citrate (Al chloride plus excess sodium citrate). Al levels were measured in plasma, urine and liver by atomic absorption spectroscopy (AAS). Liver and kidney Al content was measured at the cellular and subcellular level by electron probe X-ray microanalysis (EPXMA).

2 Urinary excretion of Al was significantly higher (P < 0.01), when Al was given as the citrate than as the chloride. After 1h, plasma Al levels were significantly lower in the Al citrate group than the Al chloride group (59 ± 3.7 vs 877 ± 214 nmol ml^-1, respectively; P< 0.01).

3 Al concentrations were significantly higher in the livers of rats receiving Al chloride (818 ± 252 nmol g^-1 wet weight; P < 0.05), than in either control or Al citrate groups (122 ± 41 and 107 ± 26 nmol g^-1 wet weight, respectively). Al concentrations derived from EPXMA measurements were in agreement with AAS values for the three groups, with significantly higher Al concentra tions in the Al chloride group (1.7 ± 0.4 nmol mg^-1 dry weight; P < 0.05) than in the control or Al citrate groups, where Al was not detectable. EPXMA analysis showed that Al was distributed in all liver organelles analysed (cytoplasm, mitochondria, nucleus, ER) and was not preferentially taken up by any one organelle in Al chloride treated rats.

4 Significant amounts of Al were found in cytoplasm and mitochondria of proximal tubule cells of rats given Al citrate (0.64 ± 0.15 and 0.80 ± 0.11 nmol mg^-1 dry weight, respectively), but not in nuclei or lysosomes of these cells. Al levels were not detectable in control kid neys, in proximal tubule cells after Al chloride adminis tration or distal tubule cells after either Al treatment.

Key Words: aluminium • citrate • liver • kidney • electron probe X-ray microanalysis • atomic absorption spectroscopy

Human & Experimental Toxicology, Vol. 14, No. 10, 787-794 (1995)
DOI: 10.1177/096032719501401002


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