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Inhibition of aflatoxin B1-induced cell injury by selenium: an in vitro study

Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore 0511

Yin-Chin Hew

Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore 0511

Choon-Nam Ong

Department of Community, Occupational and Family Medicine, National University of Singapore, Singapore 0511

Dietary selenium is an essential trace element in human nutrition. Selenium has been shown in animal studies to inhibit aflatoxin hepatocarcinogenesis. However, the cellu lar mechanism responsible for the inhibition has not been thoroughly studied. This study examines the effect of two selenium compounds, namely, sodium selenite and selenium- enriched yeast extract (SeY), on the cytotoxicity, DNA- binding and mutagenicity of aflatoxin B1 (AFB1) in cul tured Chinese hamster ovary (CHO) cells. CHO cells, after treatment with 2 µg ml^-1 selenite or 80 µg ml-1 SeY, exhibit ed increased resistance to AFB1-induced cell killing. At a concentration of 50 µg ml^-1 AFB1, cell survival, measured by the clonogenicity assay, was increased by 21- and 10- fold in selenite- and SeY-treated cells, respectively. However, selenium treatment did not appear to affect AFB1-DNA binding. Similarly, no effect was observed on AFB1 mutagenicity, as determined by the hypoxanthine- guanine phosphoribosyl transferase (HPRT) gene mutation assay. The results showed that selenium could effectively protect cells from AFB1 cytotoxicity in cultured cells but had no effect on AFB1-DNA adduct formation or mutagen esis. It is suggested that there are multiple pathways of AFB1 toxicity and that selenium can modulate AFB1- induced cell killing independent of its genotoxicity.

Key Words: aflatoxin B1 • selenium • cytotoxicity • DNA bind ing • mutagenicity

Human & Experimental Toxicology, Vol. 14, No. 1, 55-60 (1995)
DOI: 10.1177/096032719501400111


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