This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by De Paris, P. Articles by Caroldi, S. Search for Related Content PubMed PubMed Citation Articles by De Paris, P. Articles by Caroldi, S. Social Bookmarking                         What's this?

In Vivo Inhibition of Serum Dopamine-β-Hydroxylase by CaNa2 EDTA injection

Istituto di Medicina del Lavoro, University of Padova, V.Facciolati 71, I-35127 Padova, Italy

Stefano Caroldi

Istituto di Medicina del Lavoro, University of Padova, V.Facciolati 71, I-35127 Padova, Italy

1 In humans CaNa ₂EDTA increases urinary excretion of lead as well as that of essential metals such as zinc, ferrum and manganese but not that of copper.

2 We studied the effect of CaNa₂EDTA injection on serum dopamine-β-hydroxylase, a copper-dependent enzyme, in three male lead welders hospitalized for suspected lead poisoning.

3 injection of CaNa ₂EDTA (1000 mg i.v.) resulted in rapid reduction of serum dopamine-β-hydroxylase activity which returned to normal level within 24 hours.

4 Copper sulphate (8 µM) but not ethylmaleimide (10 mM) restored in vitro serum dopamine-β-hydroxylase activity to pre-dosing values which indicates that CaNa₂EDTA inhibits dopamine-β-hydroxylase by removing copper from the enzyme.

5 An inverse dose effect relationship between injected CaNa₂EDTA and residual serum dopamine-β-hydroxylase activity was observed.

6 In spite of the reported lack of changes in copper serum concentration, CaNa₂EDTA significantly affects the activity of a copper-dependent enzyme in the serum.

7 As CaNa ₂EDTA is currently the drug of choice for the treatment of asymptomatic lead poisoning in children, the effect of CaNa₂EDTA on copper-enzymes requires further investigation.

Human & Experimental Toxicology, Vol. 13, No. 4, 253-256 (1994)
DOI: 10.1177/096032719401300405


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?