This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Hiebert, L. M. Articles by Liu, J.-M. Search for Related Content PubMed PubMed Citation Articles by Hiebert, L. M. Articles by Liu, J.-M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB HYDROGEN PEROXIDE Social Bookmarking                   What's this?

Dextran Sulphates Protect Porcine Arterial Endothelial Cells from Free Radical Injury

Department of Veterinary Physiological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N 0W0

Ji-Min Liu

Department of Veterinary Physiological Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada, S7N 0W0

1 The ability of dextran sulphate to protect cultured porcine arterial endothelial cells injured by addition of xanthine and xanthine oxidase (X/XO) or hydrogen peroxide to cell medium was examined using a variety of drug preparations. Cell damage was assessed by determining cell viability (by trypan blue exclusion) and release of lactate dehydrogenase into the medium.

2 Dextran sulphates of average molecular weight (M_r) 5000, 8000 (hydrogenated or unhydrogenated) at 0.05, 0.5, 5 and 50 µg ml^-1 medium, added 24 h prior to X/XO, protected cells, whereas dextran sulphate M_r 500,000 was protective only at 0.5 µg ml^-1.

3 None of the dextran sulphates used showed any toxic effect on cells in concentrations up to 500 µg ml^-1 medium.

4 When the duration of pretreatment with dextran sulphate M_r 8000 was varied, 6 h was required for a protective effect on cells damaged by X/XO, which was enhanced with durations of 16 and 24 h.

5 Dextran sulphates had a similar protective effect on cells damaged by hydrogen peroxide.

6 This study suggests that dextran sulphates may prevent conditions resulting from free radical injury.

Human & Experimental Toxicology, Vol. 13, No. 4, 233-239 (1994)
DOI: 10.1177/096032719401300402


CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?